{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T20:55:14Z","timestamp":1715547314089},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T00:00:00Z","timestamp":1233273600000},"content-version":"vor","delay-in-days":3348,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Effects of K<jats:sup>+<\/jats:sup> channel opener, levcromakalim, on vascular endothelial cells were examined. Under voltage\u2010 and current\u2010clamp conditions, application of acetylcholine to dispersed endothelial cells isolated from rabbit superior mesenteric artery (dispersed RMAECs) produced hyperpolarization and outward currents. On the other hand, dispersed RMAECs did not respond to levcromakalim.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>When membrane potential was recorded from endothelium in a mesenteric arterial segment, exposure to levcromakalim in a concentration range of 0.1 to 3\u2003\u03bc<jats:sc>M<\/jats:sc> caused concentration\u2010dependent hyperpolarization. The hyperpolarization was observed in the absence of external Ca<jats:sup>2+<\/jats:sup> and was inhibited by 10\u2003\u03bc<jats:sc>M<\/jats:sc> glibenclamide.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The presence of 1\u2003m<jats:sc>M<\/jats:sc> heptanol did not affect the levcromakalin\u2010induced hyperpolarization, whereas treatment of the mesenteric arterial segment with 20\u2003\u03bc<jats:sc>M<\/jats:sc> 18 \u03b2\u2010glycyrrhetinic acid significantly reduced the hyperpolarization. The response to acetylcholine of RMAECs in an arterial segment with 18 \u03b2\u2010glycyrrhetinic acid was, however, similar to that without 18 \u03b2\u2010glycyrrhetinic acid.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These suggest that although RMAECs themselves are functionally insensitive to levcromakalim, those in an arterial segment are hyperpolarized by levcromakalim <jats:italic>via<\/jats:italic> myo\u2010endothelial electrical communication.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>128<\/jats:bold>, 1491\u20131496; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702956\">10.1038\/sj.bjp.0702956<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702956","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:28:56Z","timestamp":1106944136000},"page":"1491-1496","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Levcromakalim causes indirect endothelial hyperpolarization <i>via<\/i> a myo\u2010endothelial pathway"],"prefix":"10.1111","volume":"128","author":[{"given":"Takeshi","family":"Murai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuhiko","family":"Muraki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Imaizumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.561bq.x"},{"key":"e_1_2_7_3_1","first-page":"1104","article-title":"Glycyrrhetinic acid derivatives: a novel class of inhibitors of gap\u2010junctional intercellular communication. Structure\u2010activity relationships","volume":"246","author":"DAVIDSON J.S.","year":"1988","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.2967"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199607)16:3<157::AID-MC6>3.0.CO;2-E"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1999.276.6.L1018"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.319bn.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017565"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.3.C812"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020755"},{"key":"e_1_2_7_11_1","first-page":"H2462","article-title":"ATP\u2010sensitive potassium channels in cultured arterial segments","volume":"271","author":"KLEPPISCH T.","year":"1996","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_12_1","first-page":"387","article-title":"Pharmacological and physiological significance of ion channels and factors that modulate them in vascular tissues","volume":"47","author":"KURIYAMA H.","year":"1995","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.397bk.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050536"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3300695"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00178979"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00392067"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15272.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(90)90633-H"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018667"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6949"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701140"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.59.1.145"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13861.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.5.C1112"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1997.77.4.1165"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.258.2.C195"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240051063"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00960-1"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702078"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.501bh.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.505ae.x"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0702956","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0702956","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0702956","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T16:49:30Z","timestamp":1698511770000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0702956"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,12]]},"references-count":31,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1999,12]]}},"alternative-id":["10.1038\/sj.bjp.0702956"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0702956","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,12]]},"assertion":[{"value":"2009-01-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}