{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:12:52Z","timestamp":1761675172249},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T00:00:00Z","timestamp":1233273600000},"content-version":"vor","delay-in-days":3317,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We have previously shown that ATP increased cyclic AMP in NG108\u201015 cells, which was inhibited by P<jats:sub>1<\/jats:sub> receptor antagonist methylxanthines. In the present study, we examined the effects of P<jats:sub>1<\/jats:sub> and P2 receptor antagonists on cyclic AMP formation induced by \u03b2,\u03b3\u2010methyleneATP (\u03b2,\u03b3\u2010MeATP) and CGS21680, an A<jats:sub>2A<\/jats:sub> adenosine receptor agonist, in NG108\u201015 cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>\u03b2,\u03b3\u2010MeATP and CGS21680 increased intracellular cyclic AMP with EC<jats:sub>50<\/jats:sub> values of 8.0\u00b10.98\u2003\u03bc<jats:sc>M<\/jats:sc> (<jats:italic>n<\/jats:italic>=4) and 42\u00b17.5\u2003n<jats:sc>M<\/jats:sc> (<jats:italic>n<\/jats:italic>=4), respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Several P<jats:sub>1<\/jats:sub> receptor antagonists inhibited both \u03b2,\u03b3\u2010MeATP\u2010 and CGS21680\u2010induced cyclic AMP increase with a similar rank order of potency; ZM241385&gt;CGS15943&gt;XAC&gt;DPCPX. However, the pK<jats:sub>i<\/jats:sub> values of these antagonists for \u03b2,\u03b3\u2010MeATP were larger than those for CGS21680.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Alloxazine, a P<jats:sub>1<\/jats:sub> receptor antagonist, and several P2 receptor antagonists (PPADS, <jats:italic>i<\/jats:italic>PPADS, reactive blue\u20102) inhibited \u03b2,\u03b3\u2010MeATP\u2010induced response, while these antagonists little affected CGS21680\u2010induced one. Suramin was effective only for \u03b2,\u03b3\u2010MeATP\u2010induced response at 1\u2003m<jats:sc>M<\/jats:sc>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>2\u2010chloroadenosine (2CADO) and 2\u2010chloroATP (2ClATP) increased cyclic AMP with similar potencies. The effects of these agonists were both inhibited by ZM241385, but only 2ClATP\u2010induced response was inhibited by PPADS.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>ATP\u2010 and \u03b2,\u03b3\u2010MeATP\u2010induced responses were little affected by \u03b1,\u03b2\u2010methyleneADP, a 5\u2032\u2010nucleotidase inhibitor.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results clearly demonstrate that ATP\u2010stimulated cyclic AMP formation can be distinguished from the A<jats:sub>2A<\/jats:sub> receptor agonist\u2010induced one by using the several P<jats:sub>1<\/jats:sub> and P2 receptor antagonists.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>129<\/jats:bold>, 291\u2013298; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703053\">10.1038\/sj.bjp.0703053<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703053","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:40:58Z","timestamp":1106944858000},"page":"291-298","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effects of P<sub>1<\/sub> and P2 receptor antagonists on <i>\u03b2,\u03b3<\/i>\u2010methyleneATP\u2010 and CGS21680\u2010induced cyclic AMP formation in NG108\u201015 cells"],"prefix":"10.1111","volume":"129","author":[{"given":"Satoko","family":"Ohkubo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junko","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironori","family":"Nakanishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Matsuoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb14087.x"},{"key":"e_1_2_7_3_1","first-page":"1238","article-title":"ATP inhibits the synaptic release of acetylcholine in submucosal neurons","volume":"274","author":"BARAJAS\u2010L\u00d3PEZ C.","year":"1995","journal-title":"J. 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