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Concentrations of 300\u2003\u03bc<jats:sc>M<\/jats:sc> or less of <jats:sc>L<\/jats:sc>\u2010cysteine had no effect on responses.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Relaxations produced by exogenous NO<jats:sup>\u2022<\/jats:sup> (0.25\u20132.5\u2003\u03bc<jats:sc>M<\/jats:sc>) were markedly prolonged and relaxations produced by sodium nitroprusside (0.001\u20130.3\u2003\u03bc<jats:sc>M<\/jats:sc>) were enhanced by 1 and 3\u2003m<jats:sc>M<\/jats:sc> <jats:sc>L<\/jats:sc>\u2010cysteine. The enhancements by <jats:sc>L<\/jats:sc>\u2010cysteine of responses to NO<jats:sup>\u2022<\/jats:sup> and sodium nitroprusside may be attributed to the formation of S\u2010nitrosocysteine.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Relaxations mediated by the nitroxyl anion (0.3\u2003\u03bc<jats:sc>M<\/jats:sc>) donated from Angeli's salt were more prolonged than those produced by NO<jats:sup>\u2022<\/jats:sup>, and nitroxyl\u2010induced relaxations were reduced by <jats:sc>L<\/jats:sc>\u2010cysteine (1 and 3\u2003m<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>EDRF\u2010mediated relaxations produced by acetylcholine (0.01\u201310\u2003\u03bc<jats:sc>M<\/jats:sc>), ATP (3\u2013100\u2003\u03bc<jats:sc>M<\/jats:sc>) and the calcium ionophore A23187 (0.1\u2003\u03bc<jats:sc>M<\/jats:sc>) were significantly reduced by 3\u2003m<jats:sc>M<\/jats:sc> <jats:sc>L<\/jats:sc>\u2010cysteine.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The similarity between the inhibitory effects of <jats:sc>L<\/jats:sc>\u2010cysteine on responses to EDRF and on those to nitroxyl suggests that a component of the response to EDRF may be mediated by nitroxyl anion.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>129<\/jats:bold>, 315\u2013322; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703058\">10.1038\/sj.bjp.0703058<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703058","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:40:58Z","timestamp":1106944858000},"page":"315-322","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Differential actions of L\u2010cysteine on responses to nitric oxide, nitroxyl anions and EDRF in the rat aorta"],"prefix":"10.1111","volume":"129","author":[{"given":"Anthie","family":"Ellis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Guang Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J","family":"Rand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00054a013"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.11.6041"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(92)90133-O"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.33.227"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)90640-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702300"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041230304"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(96)00078-0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(97)01269-7"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00210a047"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702374"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/368062a0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01928788"},{"key":"e_1_2_7_15_1","first-page":"546","article-title":"The pharmacological activity of nitroxyl: a potent vasodilator with activity similar to nitric oxide and\/or endothelium\u2010derived relaxing factor","volume":"263","author":"FUKUTO J.M.","year":"1992","journal-title":"J. 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