{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T18:52:40Z","timestamp":1785178360601,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T00:00:00Z","timestamp":1233273600000},"content-version":"vor","delay-in-days":3286,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The permeabilizing effect of P2X<jats:sub>7<\/jats:sub> agonists was tested in rat submandibular acinar cells using the uptake of ethidium bromide as an index.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The uptake of ethidium bromide by acini incubated at 37\u00b0C in the presence of 1\u2003m<jats:sc>M<\/jats:sc> ATP increased with time and reached after 5\u2003min about 10% of maximal uptake measured in the presence of digitonin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The response to ATP was dose\u2010dependent (half\u2010maximal concentration around 40\u2003\u03bc<jats:sc>M<\/jats:sc>) and it was decreased when the temperature was lowered to 25\u00b0C.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Benzoyl\u2010ATP reproduced the response to ATP (half\u2010maximal concentration around 10\u2003\u03bc<jats:sc>M<\/jats:sc>). UTP or 2\u2010methylthioATP had no effect.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The permeabilization in response to ATP was blocked by oxidized ATP and by magnesium and inhibited by Coomassie blue.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>ATP increased the activity of a calcium\u2010insensitive phospholipase A<jats:sub>2<\/jats:sub> (iPLA<jats:sub>2<\/jats:sub>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Bromoenol lactone (BEL) inhibited the iPLA<jats:sub>2<\/jats:sub> stimulated by ATP but potentiated the uptake of ethidium bromide in response to the purinergic agonist.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>From these results it is concluded that the activation of P2X<jats:sub>7<\/jats:sub> receptors permeabilizes rat submandibular acinar cells. The pore\u2010forming activity of the receptor might be negatively regulated by the concomitant activation of the iPLA<jats:sub>2<\/jats:sub> by the receptor.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>129<\/jats:bold>, 703\u2013708; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703124\">10.1038\/sj.bjp.0703124<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703124","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:46:01Z","timestamp":1106945161000},"page":"703-708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Bromoenol lactone enhances the permeabilization of rat submandibular acinar cells by P2X<sub>7<\/sub> agonists"],"prefix":"10.1111","volume":"129","author":[{"given":"N","family":"Cha\u00efb","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Kabr\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Alzola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Pochet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J P","family":"Dehaye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.46.30208"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.5.C1546"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.18.8527"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"3193","DOI":"10.1074\/jbc.271.50.31937","article-title":"Bromoenol lactone inhibits magnesium\u2010dependent phosphatidate phosphohydrolase and blocks triacylglycerol biosynthesis in mouse P388D1 macrophages","volume":"271","author":"BALSINDE J.","year":"1996","journal-title":"J. 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