{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:05:23Z","timestamp":1791266723965,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":3260,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>This study examines the relationship between \u03b2<jats:sub>3a<\/jats:sub>\u2010 and \u03b2<jats:sub>3b<\/jats:sub>\u2010adrenoceptor (AR) mRNA levels, \u03b2<jats:sub>3<\/jats:sub>\u2010AR binding and changes in ileum responses in mice treated with the \u03b2<jats:sub>3<\/jats:sub>\u2010AR agonist (<jats:bold>R,R<\/jats:bold>)\u20105\u2010[2[[2\u2010(3\u2010chlorophenyl)\u20102\u2010hydroxyethyl]\u2010amino]\u2010propyl]1,3\u2010benzodioxole\u20102,2\u2010dicarboxylate (CL316243), or the \u03b2<jats:sub>3<\/jats:sub>\u2010AR antagonist 3\u2010(2\u2010ethylphenoxy)\u20101\u2010[(1<jats:bold>S<\/jats:bold>)\u20101,2,3,4\u2010tetrahydronapth\u20101\u2010ylamino]\u20102<jats:bold>S<\/jats:bold>\u20102\u2010propanol oxalate (SR59230A), or dexamethasone or forskolin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Levels of \u03b2<jats:sub>3a<\/jats:sub>\u2010 and \u03b2<jats:sub>3b<\/jats:sub>\u2010AR mRNA and the maximum number of binding sites (B<jats:sub>max<\/jats:sub>) in ileum were unaffected following CL316243 treatment, although responses to CL316243 were reduced by 50% following 4 and 24\u2003h treatment, indicating another desensitization mechanism not involving changes in receptor expression or number. \u03b2<jats:sub>3a<\/jats:sub>\u2010AR mRNA levels were reduced in both brown (BAT) and white adipose tissue (WAT) but \u03b2<jats:sub>3b<\/jats:sub>\u2010AR mRNA levels were significantly reduced only in WAT. Levels of \u03b2<jats:sub>3a<\/jats:sub>\u2010 and \u03b2<jats:sub>3b<\/jats:sub>\u2010mRNA returned towards normal with continued treatment.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>SR59230A treatment markedly increased \u03b2<jats:sub>3<\/jats:sub>\u2010AR mRNA levels in ileum and BAT but not in WAT. The increase in \u03b2<jats:sub>3<\/jats:sub>\u2010AR mRNA levels in ileum was associated with increased B<jats:sub>max<\/jats:sub> levels in binding analysis and increased responses to CL316243, suggesting these as the cause of sensitization.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Treatment with forskolin (4\u2003h) or dexamethasone (4\u2003h) significantly reduced \u03b2<jats:sub>3a<\/jats:sub>\u2010AR mRNA levels in BAT and WAT but did not alter levels in ileum. Responses to CL316243 in ileum were unaffected by either treatment.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In summary, the \u03b2<jats:sub>3<\/jats:sub>\u2010AR is differently regulated in adipose tissue and ileum: Treatment with SR59230A increased \u03b2<jats:sub>3<\/jats:sub>\u2010AR number, mRNA and responsiveness in ileum, whereas treatment with CL316243 reduced responses without affecting \u03b2<jats:sub>3<\/jats:sub>\u2010AR number or mRNA levels.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>129<\/jats:bold>, 1251\u20131259; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703160\">10.1038\/sj.bjp.0703160<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703160","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:34:44Z","timestamp":1106944484000},"page":"1251-1259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<i>\u03b2<\/i><sub>3<\/sub>\u2010Adrenoceptor regulation and relaxation responses in mouse ileum"],"prefix":"10.1111","volume":"129","author":[{"given":"Dana S","family":"Hutchinson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bronwyn A","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger J","family":"Summers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/309163a0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.52.33366"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)80516-W"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1979.59.3.719"},{"key":"e_1_2_7_6_1","first-page":"40P","article-title":"\u03b2\u2010Adrenoceptors in guinea\u2010pig gastric fundus\u2013are they the same as the \u2018atypical\u2019\u03b2\u2010adrenoceptors in rat adipocytes","volume":"90","author":"COLEMAN R.A.","year":"1987","journal-title":"Br. 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