{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:55:33Z","timestamp":1774439733810,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":3229,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effects of \u03b2<jats:sub>3<\/jats:sub>\u2010adrenergic stimulation were studied on the L\u2010type Ca<jats:sup>2+<\/jats:sup> channel in single myocytes from rat portal vein using the whole\u2010cell mode of the patch\u2010clamp technique.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Reverse transcription\u2010polymerase chain reaction showed that \u03b2<jats:sub>1<\/jats:sub>\u2010, \u03b2<jats:sub>2<\/jats:sub>\u2010 and \u03b2<jats:sub>3<\/jats:sub>\u2010adrenoceptor subtypes were expressed in rat portal vein myocytes. Application of both propranolol (a non\u2010selective \u03b2<jats:sub>1<\/jats:sub>\u2010 and \u03b2<jats:sub>2<\/jats:sub>\u2010adrenoceptor antagonist) and SR59230A (a \u03b2<jats:sub>3<\/jats:sub>\u2010adrenoceptor antagonist) were needed to inhibit the isoprenaline\u2010induced increase in L\u2010type Ca<jats:sup>2+<\/jats:sup> channel current.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>L\u2010type Ca<jats:sup>2+<\/jats:sup> channels were stimulated by CGP12177A (a \u03b2<jats:sub>3<\/jats:sub>\u2010adrenoceptor agonist with potent \u03b2<jats:sub>1<\/jats:sub>\u2010 and \u03b2<jats:sub>2<\/jats:sub>\u2010adrenoceptor antagonist property) in a manner similar to that of isoprenaline. The CGP12177A\u2010induced stimulation of Ca<jats:sup>2+<\/jats:sup> channel current was blocked by SR59230A, cyclic AMP\u2010dependent protein kinase inhibitors, H\u201089 and Rp 8\u2010Br\u2010cyclic AMPs, but was unaffected by protein kinase C inhibitors, GF109203X and 19\u201031 peptide. This stimulation was mimicked by forskolin and 8\u2010Br\u2010cyclic AMP. In the presence of okadaic acid (a phosphatase inhibitor), the \u03b2<jats:sub>3<\/jats:sub>\u2010adrenoceptor\u2010induced stimulation was maintained after withdrawal of the agonist.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The \u03b2<jats:sub>3<\/jats:sub>\u2010adrenoceptor stimulation of L\u2010type Ca<jats:sup>2+<\/jats:sup> channels was blocked by a pretreatment with cholera toxin and by the intracellular application of an anti\u2010G\u03b1<jats:sub>s<\/jats:sub> antibody. This stimulation was unaffected by intracellular infusion of an anti\u2010G\u03b2<jats:sub>com<\/jats:sub> antibody and a \u03b2ARK<jats:sub>1<\/jats:sub> peptide.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results show that activation of \u03b2<jats:sub>3<\/jats:sub>\u2010adrenoceptors stimulates L\u2010type Ca<jats:sup>2+<\/jats:sup> channels in vascular myocytes through a G\u03b1<jats:sub>s<\/jats:sub>\u2010induced stimulation of the cyclic AMP\/protein kinase A pathway and the subsequent phosphorylation of the channels.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>129<\/jats:bold>, 1497\u20131505; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703187\">10.1038\/sj.bjp.0703187<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703187","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:35:19Z","timestamp":1106944519000},"page":"1497-1505","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Beta\u20103 adrenergic stimulation of <scp>L<\/scp>\u2010Type Ca<sup>2+<\/sup> channels in rat portal vein myocytes"],"prefix":"10.1111","volume":"129","author":[{"given":"Patricia","family":"Viard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Macrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Coussin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Luc","family":"Morel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Mironneau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.1998.00345.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(97)90024-5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.11.1019"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-74-10-1149"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0898-6568(94)90093-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.42.25259"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.003bx.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2570461"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)61994-3"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb15852.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701523"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118823"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.55.2.255"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.73.6.1128"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(96)01033-4"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1124\/mol.53.4.670","article-title":"(\u2212)\u2010CGP12177 caused cardiostimulation and binds to cardiac putative \u03b24\u2010adrenoceptors in both wild\u2010type and \u03b23\u2010adrenoceptor knockout mice","volume":"53","author":"KAUMANN A.J.","year":"1998","journal-title":"Mol. 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