{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T08:26:00Z","timestamp":1759047960076},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":3229,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The increase in the cytosolic Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) following repetitive stimulation with ATP or sphingosylphosphorylcholine (SPC) in single porcine aortic smooth muscle cells was investigated using the Ca<jats:sup>2+<\/jats:sup> indicator, fura\u20102.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The ATP\u2010induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase resulted from both Ca<jats:sup>2+<\/jats:sup> release and Ca<jats:sup>2+<\/jats:sup> influx. The former was stimulated by phospholipase\u2003C activation, while the latter occurred predominantly <jats:italic>via<\/jats:italic> the receptor\u2010operated Ca<jats:sup>2+<\/jats:sup> channels (ROC), rather than the store\u2010operated Ca<jats:sup>2+<\/jats:sup> channels (SOC) or the voltage\u2010operated Ca<jats:sup>2+<\/jats:sup> channel (VOC). Furthermore, the P2X<jats:sub>5<\/jats:sub> receptor was shown to be responsible for the ATP\u2010induced Ca<jats:sup>2+<\/jats:sup> influx.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>A reproducible [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase was induced by repetitive ATP stimulation, but was abolished by removal of extracellular Ca<jats:sup>2+<\/jats:sup> or inhibition of intracellular Ca<jats:sup>2+<\/jats:sup> release using U\u201073122 or thapsigargin, and was restored by Ca<jats:sup>2+<\/jats:sup> readdition in the former case.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>SPC only caused Ca<jats:sup>2+<\/jats:sup> release, and the amplitude of the repetitive SPC\u2010induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increases declined gradually. However, a reproducible [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase was seen in cells in which protein kinase\u2003C being inhibited, which increased the SPC\u2010induced Ca<jats:sup>2+<\/jats:sup> influx, rather than IP<jats:sub>3<\/jats:sub> generation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, although the amplitude of the ATP\u2010induced Ca<jats:sup>2+<\/jats:sup> release, measured when Ca<jats:sup>2+<\/jats:sup> influx was blocked, or of the Ca<jats:sup>2+<\/jats:sup> influx when Ca<jats:sup>2+<\/jats:sup> release was blocked, progressively decreased following repetitive stimulation, the overall [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase for each stimulation under physiological conditions remained the same, suggesting that the Ca<jats:sup>2+<\/jats:sup> stores were replenished by an influx of Ca<jats:sup>2+<\/jats:sup> during stimulation. The SPC\u2010induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase resulted solely from Ca<jats:sup>2+<\/jats:sup> release and decreased gradually following repetitive stimulation, but the decrease could be prevented by stimulating Ca<jats:sup>2+<\/jats:sup> influx, further supporting involvement of the intracellular Ca<jats:sup>2+<\/jats:sup> stores in Ca<jats:sup>2+<\/jats:sup> signalling.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>129<\/jats:bold>, 1365\u20131374; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703190\">10.1038\/sj.bjp.0703190<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703190","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:35:19Z","timestamp":1106944519000},"page":"1365-1374","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Distinct Ca<sup>2+<\/sup> signalling mechanisms induced by ATP and sphingosylphosphorylcholine in porcine aortic smooth muscle cells"],"prefix":"10.1111","volume":"129","author":[{"given":"Ting\u2010Yu","family":"Chin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheau\u2010Huei","family":"Chueh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(96)10041-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021927"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.26.15195"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(98)01170-5"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.5.C1255"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"13883","DOI":"10.1016\/S0021-9258(18)66953-4","article-title":"Inositol 1,4,5\u2010trisphosphate and guanine nucleotides activate calcium release from endoplasmic reticulum via distinct mechanisms","volume":"261","author":"CHUEH S.H.","year":"1986","journal-title":"J. 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