{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:18:16Z","timestamp":1762100296754},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":3229,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The current study explored potential redox mechanisms of nitric oxide (NO)\u2010induced inhibition of DNA synthesis in cultured human and rat aortic smooth muscle cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Exposure to S\u2010nitrosothiols, DETA\u2010NONOate and NO itself inhibited ongoing DNA synthesis and S phase progression in a concentration\u2010dependent manner, as measured by thymidine incorporation and flow cytometry. Inhibition by NO donors occurred by release of NO, as detected by chemiluminescence and judged by the effects of NO scavengers, haemoglobin and cPTIO.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Co\u2010incubation with redox compounds, N\u2010acetyl\u2010<jats:sc>L<\/jats:sc>\u2010cysteine, glutathione and <jats:sc>L<\/jats:sc>\u2010ascorbic acid prevented NO inhibition of DNA synthesis. These observations suggest that redox agents may alternatively attenuate NO bioactivity extracellularly, interfere with intracellular actions of NO on the DNA synthesis machinery or restore DNA synthesis after established inhibition by NO.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Recovery of DNA synthesis after inhibition by NO was similar with and without redox agents suggesting that augmented restoration of DNA synthesis is an unlikely mechanism to explain redox regulation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Study of extracellular interactions revealed that all redox agents potentiated S\u2010nitrosothiol decomposition and NO release.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Examination of intracellular NO bioactivity showed that as opposed to attenuation of NO inhibition of DNA synthesis by redox agents, there was no inhibition (potentiation in the presence of ascorbic acid) of soluble guanylate cyclase (sGC) activation judged by cyclic GMP accumulation in rat cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data provide evidence that NO\u2010induced inhibition of ongoing DNA synthesis is sensitive to redox environment. Redox processes might protect the DNA synthesis machinery from inhibition by NO, in the setting of augmented liberation of biologically active NO from NO donors.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>129<\/jats:bold>, 1513\u20131521; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703240\">10.1038\/sj.bjp.0703240<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703240","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:35:19Z","timestamp":1106944519000},"page":"1513-1521","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A redox\u2010based mechanism for nitric oxide\u2010induced inhibition of DNA synthesis in human vascular smooth muscle cells"],"prefix":"10.1111","volume":"129","author":[{"given":"Ruth E","family":"Bundy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00e1ndor","family":"Marczin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian H","family":"Chester","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdi","family":"Yacoub","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1650033"},{"key":"e_1_2_7_3_1","first-page":"1","article-title":"Radioimmunoassay of cyclic AMP and cyclic GMP","volume":"10","author":"BROOKER G.","year":"1979","journal-title":"Adv. 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