{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:40:49Z","timestamp":1771461649055,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T00:00:00Z","timestamp":1233273600000},"content-version":"vor","delay-in-days":3226,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The present study extends our previous finding that the endothelium\u2010independent relaxation in porcine coronary artery rings is enhanced after short\u2010term (20\u2003min) exposure to a physiological concentration (1\u2003n<jats:sc>M<\/jats:sc>) of 17\u03b2\u2010estradiol and demonstrates that this effect may be attributable to activation of the cyclic AMP pathway.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Isometric tension was recorded in isolated rings of porcine coronary arteries.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Relaxation by levcromakalim and sodium nitroprusside, but not bradykinin and calcium ionophore A23187, were significantly potentiated following 20\u2003min treatment with 1\u2003n<jats:sc>M<\/jats:sc> 17\u03b2\u2010estradiol. This enhancing effect was insensitive to the transcriptional and translational inhibitors, actinomycin D and cycloheximide respectively and absent following repeated washing of the rings prior to construction of relaxation\u2010response curves.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The potentiating actions of 1\u2003n<jats:sc>M<\/jats:sc> 17\u03b2\u2010estradiol on endothelium\u2010independent relaxation were mimicked by the cyclic AMP analogue 8\u2010Bromo\u2010cyclic AMP and the protein kinase A activator Sp\u2010cyclic AMPS but not by the cyclic GMP analogue 8\u2010Bromo\u2010cyclic GMP. The modulatory effect of 17\u03b2\u2010estradiol was increased in the presence of the phosphodiesterase inhibitor 3\u2010isobutyl\u20101\u2010methylxanthine.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The cyclic AMP\u2010dependent protein kinase A inhibitor Rp\u2010cyclic AMPS, but not the cyclic GMP antagonist Rp\u20108\u2010Bromo\u2010cyclic GMPS, effectively inhibited the enhancing effects 1\u2003<jats:sc>M<\/jats:sc> 17\u03b2\u2010estradiol had on the relaxation responses of levcromakalim and sodium nitroprusside.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data support our earlier findings that physiologically relevant concentrations of 17\u03b2\u2010estradiol can acutely modify vasorelaxation <jats:italic>in vitro<\/jats:italic>. Furthermore, we report that this short\u2010term effect of 17\u03b2\u2010estradiol on vasorelaxation appears to be mediated <jats:italic>via<\/jats:italic> non\u2010genomic pathways and involves the cyclic AMP cascade.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>129<\/jats:bold>, 1739\u20131747; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703252\">10.1038\/sj.bjp.0703252<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703252","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:38:28Z","timestamp":1106944708000},"page":"1739-1747","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Enhanced relaxation of porcine coronary arteries after acute exposure to a physiological level of 17\u03b2\u2010estradiol involves non\u2010genomic mechanisms and the cyclic AMP cascade"],"prefix":"10.1111","volume":"129","author":[{"given":"Hwee","family":"Teoh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricky Y K","family":"Man","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.18.8517"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.95.1.252"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.75.6.1102"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(95)05599-R"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0029-7844(95)00358-4"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(95)02159-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.90.2.786"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.89.6.2545"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"3620","DOI":"10.1523\/JNEUROSCI.16-11-03620.1996","article-title":"17\u03b2\u2010Estradiol potentiates kainate\u2010induced currents via activation of the cAMP cascade","volume":"16","author":"GU Q.","year":"1996","journal-title":"J. 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