{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:32:45Z","timestamp":1762144365114},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3195,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>To analyse the mechanisms of muscle layer\u2010dependent inhibition of porcine myometrial contractility by 5\u2010hydroxytryptamine (5\u2010HT), the effects of 5\u2010HT, 5\u2010carboxamidotryptamine(5\u2010CT), 5\u2010methoxytryptamine (5\u2010MeOT), forskolin and cyclic adenosine 3\u2032, 5\u2032\u2010monophosphate (cyclic AMP) analogues on spontaneous and stimulant\u2010induced contractions were examined in longitudinal (LM) and circular muscles (CM). In addition, accumulation of cyclic AMP by 5\u2010HT and distribution of 5\u2010HT<jats:sub>7<\/jats:sub> receptors in LM and CM layers were compared using biochemical and molecular approaches.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>5\u2010HT receptor agonists inhibited the spontaneous contractions of LM and CM (5\u2010CT&gt;5\u2010HT&gt;5\u2010MeOT), but CM was more sensitive than was LM. The inhibition by the agonists was antagonized by methiothepin (100\u2003n<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Carbachol\u2010, high\u2010K<jats:sup>+<\/jats:sup>\u2010, histamine\u2010 and Ca<jats:sup>2+<\/jats:sup>\u2010induced contractions were inhibited by 5\u2010HT with different responsivenesses (CM&gt;LM). Even in the presence of 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX), the inhibition by 5\u2010HT in the CM was still more conspicuous than that in the LM.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Compared with the CM, the inhibition of spontaneous contraction by forskolin, dibutyryl\u2010cyclic AMP and 8\u2010bromo\u2010cyclic AMP was marked in the LM.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>5\u2010HT (1\u2003n<jats:sc>M<\/jats:sc>\u20131\u2003\u03bc<jats:sc>M<\/jats:sc>) increased the cyclic AMP in both muscle layers, but the increment in the CM was higher than that in the LM whether IBMX was present or not.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>LM and CM layers contained a single class of [<jats:sup>3<\/jats:sup>H]\u20105\u2010CT binding sites with a similar <jats:italic>K<jats:sub>d<\/jats:sub><\/jats:italic> value (0.21\u20130.24\u2003n<jats:sc>M<\/jats:sc>). However, B<jats:sub>max<\/jats:sub> (5\u2010HT<jats:sub>7<\/jats:sub> receptor concentration) in the CM (120.6\u2003fmol\u2003mg<jats:sup>\u22121<\/jats:sup> protein) was higher than that in the LM (30.4\u2003fmol\u2003mg<jats:sup>\u22121<\/jats:sup> protein).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The molecular study (reverse transcription polymerase chain reaction) demonstrated the expression of 5\u2010HT<jats:sub>7<\/jats:sub> receptor mRNA in the CM was higher than that in the LM.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results suggest that the muscle layer\u2010dependent difference in inhibition by 5\u2010HT is not restricted to spontaneous contraction but applies to various contractions in the porcine myometrium. Different inhibition of the contractility by 5\u2010HT is caused by muscle layer\u2010related accumulation of cyclic AMP (CM&gt;LM), due to smooth muscle\u2010layer dependent distribution (CM&gt;LM) of 5\u2010HT<jats:sub>7<\/jats:sub> receptors.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>130<\/jats:bold>, 79\u201389; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703290\">10.1038\/sj.bjp.0703290<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703290","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:40:47Z","timestamp":1106944847000},"page":"79-89","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Smooth muscle layer\u2010dependent distribution of 5\u2010hydroxytryptamine<sub>7<\/sub> receptor in the porcine myometrium"],"prefix":"10.1111","volume":"130","author":[{"given":"Takio","family":"Kitazawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuko","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetomo","family":"Iwano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Yokota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Yuasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Taneike","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49479-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(94)90059-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(95)00195-Q"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(73)90196-2"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0700921"},{"key":"e_1_2_7_7_1","first-page":"157","article-title":"International union of pharmacology classification of receptors for 5\u2010hydroxytryptamine (serotonin)","volume":"46","author":"HOYER D.","year":"1994","journal-title":"Pharmacol. 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