{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T03:04:20Z","timestamp":1774494260624,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3195,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Serotonin (5\u2010hydroxytryptamine, 5\u2010HT) has been shown to increase cyclic AMP production in dispersed cell aggregates from the major salivary glands of the rat. The goal of the present study was to identify the 5\u2010HT receptor subtypes that mediate these effects in rat submandibular glands (SMG).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Among the 5\u2010HT receptor subtypes identified in the rat, 5\u2010HT<jats:sub>4(a,b)<\/jats:sub>, 5\u2010HT<jats:sub>6<\/jats:sub> and 5\u2010HT<jats:sub>7(a,b,c)<\/jats:sub> activate adenylyl cyclase (AC). We used subtype specific primers to screen rat SMG by reverse transcription\u2010PCR. Results indicate the presence of mRNA for 5\u2010HT<jats:sub>4(b)<\/jats:sub> and 5\u2010HT<jats:sub>7(a)<\/jats:sub> but not for 5\u2010HT<jats:sub>4(a)<\/jats:sub>, 5\u2010HT<jats:sub>6<\/jats:sub> and 5\u2010HT<jats:sub>7(b,c)<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In dispersed SMG cells, 5\u2010carboxyamidotryptamine (5\u2010CT), a 5\u2010HT<jats:sub>7<\/jats:sub> receptor agonist, stimulated cyclic AMP synthesis with higher potency (EC<jats:sub>50<\/jats:sub>=27\u00b15\u2003n<jats:sc>M<\/jats:sc>) but lower efficacy than 5\u2010HT, suggesting a 5\u2010HT<jats:sub>7<\/jats:sub> component and an additional component in the response to 5\u2010HT. The 5\u2010HT<jats:sub>7<\/jats:sub> contribution was further supported by antagonism of the 5\u2010CT effect by metergoline, a 5\u2010HT<jats:sub>7<\/jats:sub> antagonist, which exhibited an affinity (K<jats:sub>i<\/jats:sub>=50\u2003n<jats:sc>M<\/jats:sc>) similar to that obtained at the cloned 5\u2010HT<jats:sub>7<\/jats:sub> receptor.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the presence of a maximally effective concentration of 5\u2010CT, 5\u2010HT produced an additional increase in cyclic AMP production that was inhibited by the 5\u2010HT<jats:sub>4<\/jats:sub> receptor antagonist, GR113808, suggesting that the second component of cyclic AMP production is mediated by 5\u2010HT<jats:sub>4<\/jats:sub> receptors.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These findings indicate the presence in rat SMG of both 5\u2010HT<jats:sub>4(b)<\/jats:sub> and 5\u2010HT<jats:sub>7(a)<\/jats:sub> receptors positively coupled to AC.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>130<\/jats:bold>, 104\u2013108; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703303\">10.1038\/sj.bjp.0703303<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703303","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:40:47Z","timestamp":1106944847000},"page":"104-108","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of the adenylyl cyclase\u2010activating 5\u2010hydroxytryptamine receptor subtypes expressed in the rat submandibular gland"],"prefix":"10.1111","volume":"130","author":[{"given":"D M","family":"Bourdon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J M","family":"Camden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L A","family":"Landon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F O","family":"Levy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J T","family":"Turner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1981.tb16809.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(94)90059-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(95)00195-Q"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345890680010901"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(89)90177-6"},{"key":"e_1_2_7_7_1","first-page":"880","article-title":"A nonclassical 5\u2010hydroxytryptamine receptor positively coupled with adenylate cyclase in the central nervous system","volume":"34","author":"DUMUIS A.","year":"1988","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(97)01043-2"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07280.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13617.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1096\/fasebj.10.12.8903510","article-title":"Peripheral 5\u2010HT4 receptors","volume":"10","author":"HEGDE S.S.","year":"1996","journal-title":"FASEB J."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68041372.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(98)00070-7"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-4328(96)00109-X"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701336"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020230"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(00)89034-3"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00005375"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01952223"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"C1388","DOI":"10.1152\/ajpcell.1997.272.4.C1388","article-title":"Changes in P2Y1 nucleotide receptor activity during the development of rat salivary glands","volume":"272","author":"PARK M.K.","year":"1997","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_21_1","first-page":"229","article-title":"Molecular cloning of a mammalian serotonin receptor that activates adenylate cyclase","volume":"44","author":"PLASSAT J.L.","year":"1993","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1619"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.18.8547"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(94)00005-N"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46830-X"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00964-2"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701134"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1994.63020456.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(90)90170-F"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345960750031101"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701260"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0703303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0703303","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0703303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T13:22:03Z","timestamp":1698585723000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0703303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["10.1038\/sj.bjp.0703303"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0703303","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}