{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T05:16:59Z","timestamp":1698643019969},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3195,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We examined the interaction between rat\u2010amylin and relaxations induced by rat\u2010\u03b1CGRP and isoprenaline in rat isolated coronary small arteries.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Amylin, 0.1\u2013100\u2003n<jats:sc>M<\/jats:sc>, had a concentration dependent non\u2010competitive antagonistic effect on rat\u2010\u03b1CGRP\u2010induced responses with an EC<jats:sub>50<\/jats:sub> of approximately 1\u2003n<jats:sc>M<\/jats:sc>. Amylin did not affect the relaxations induced by isoprenaline at a concentration of 10\u2003n<jats:sc>M<\/jats:sc>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The apparent equilibrium dissociation constant, K<jats:sub>A<\/jats:sub>, for CGRP<jats:sub>1<\/jats:sub>\u2010receptors in the rat coronary small arteries was approximately 2\u2003n<jats:sc>M<\/jats:sc>. Analysis of the relationship between receptor occupancy and response to rat\u2010\u03b1CGRP indicates that the receptor reserve is small.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Our results show that amylin in low concentrations acts as a selective non\u2010competitive inhibitor at CGRP<jats:sub>1<\/jats:sub>\u2010receptors in rat isolated coronary small arteries.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>130<\/jats:bold>, 386\u2013390; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703316\">10.1038\/sj.bjp.0703316<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703316","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:53:27Z","timestamp":1106945607000},"page":"386-390","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Non\u2010competitive antagonism of amylin on CGRP<sub>1<\/sub>\u2010receptors in rat coronary small arteries"],"prefix":"10.1111","volume":"130","author":[{"given":"Majid","family":"Sheykhzade","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niels C Berg","family":"Nyborg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1139\/y95-144"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3018932"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(92)90106-Y"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00634231"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1989.tb08565.x"},{"key":"e_1_2_7_7_1","first-page":"21","volume-title":"Advances in Drug Research","author":"FURCHGOTT R.F.","year":"1966"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1967.tb53817.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1056-8727(96)00002-5"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/140174398427965"},{"key":"e_1_2_7_11_1","volume-title":"Pharmacological analysis of drug\u2010receptor interaction","author":"KENAKIN T.","year":"1997"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(92)90483-K"},{"key":"e_1_2_7_13_1","first-page":"290","article-title":"Alpha adrenoceptor number limits response of some rabbit arteries to norepinephrine","volume":"233","author":"LAHER I.","year":"1985","journal-title":"J. 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