{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T10:56:05Z","timestamp":1777114565045,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3134,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Carrier mediated uptake (uptake\u20101) transport of norepinephrine (NE) plays a key role in the regulation of sympathetic neurotransmission. Recent investigations indicate that nitric oxide (NO) may modulate uptake\u20101 activity, possibly in a cyclic GMP independent manner.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Carrier mediated transport of [<jats:sup>3<\/jats:sup>H\u2010NE] and [<jats:sup>3<\/jats:sup>H\u2010dopamine, DA] was examined in CHO cells transfected with cDNA for the NE and DA transporters (NET, DAT) respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>While exposure to the NO donor S\u2010nitroso\u2010N\u2010acetylpenicillamine (100\u2003\u03bc<jats:sc>M<\/jats:sc>, SNAP) significantly reduced [<jats:sup>3<\/jats:sup>H\u2010NE] uptake (<jats:italic>P<\/jats:italic>&lt;0.001), no effect on [<jats:sup>3<\/jats:sup>H\u2010DA] transport was apparent.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Comparison of the amino acid sequences for NET and DAT identified cysteine residue 351 in NET, which was not present in DAT. Site\u2010directed mutagenesis of Cys 351 to Ser produced a functional NET that was resistant to the inhibitory effects of SNAP.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The presence of SNAP mediated nitrosylation of the cysteine residue in an 8\u2010mer model peptide based around Cys 351 in NET was confirmed by both biochemical and mass spectroscopic means.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data indicate the potential regulatory role for NO in modulating sympathetic neurotransmission, and further confirm the importance of non\u2010cyclic GMP dependent mechanisms in mediating the actions of NO.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p> <jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>130<\/jats:bold>, 1060\u20131064; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703416\">10.1038\/sj.bjp.0703416<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703416","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:15:45Z","timestamp":1106946945000},"page":"1060-1064","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Nitric oxide mediated modulation of norepinephrine transport: identification of a potential target for S\u2010nitrosylation"],"prefix":"10.1111","volume":"130","author":[{"given":"David M","family":"Kaye","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Gruskin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A Ian","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murray D","family":"Esler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.16.030193.000445"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.196.1.263"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)60717-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)60723-1"},{"key":"e_1_2_7_6_1","first-page":"1030","article-title":"Structural domains of catecholamine transporter chimeras involved in selective inhibition by antidepressants and psychomotor stimulants","volume":"48","author":"BUCK K.J.","year":"1995","journal-title":"Mol. 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