{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:10:10Z","timestamp":1745367010049,"version":"3.40.4"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3103,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>While the expression and\/or activity of endothelial nitric oxide synthase (eNOS) has been characterized in spontaneously hypertensive (SHR) and normotensive Wistar Kyoto rat (WKY) hearts, in coronary endothelial cells (ECs) from both strains, the effect of NO on intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) is still unknown.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Coronary microvascular ECs were isolated from SHR and WKY and characterized. Immunocytochemistry and Western blot analysis showed that eNOS was similarly expressed in ECs from both strains.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Measuring [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> by imaging analysis of fura\u20102\u2010loaded cells, we demonstrated that \u03b1\u2010thrombin (3\u2212180\u2003U\u2003l<jats:sup>\u22121<\/jats:sup>) induced a superimposable dose\u2010dependent calcium transient in ECs from both strains.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In WKY ECs, S\u2010nitroso\u2010N\u2010acetyl\u2010DL\u2010penicillamine (SNAP) dose\u2010dependently (10\u2013100\u2003\u03bc<jats:sc>M<\/jats:sc>) and 0.1\u2003\u03bc<jats:sc>M<\/jats:sc> atrial natriuretic factor (ANF) reduced the maximum and the decay time of \u03b1\u2010thrombin\u2010induced calcium transient. The inhibitory effects of SNAP and ANF were prevented by blocking cyclic GMP\u2010dependent protein kinase. Non selective eNOS inhibitors prolonged the decay time of \u03b1\u2010thrombin\u2010induced calcium transient, while the selective inducible NOS inhibitor 1400\u2003W was ineffective. SNAP (100\u2003\u03bc<jats:sc>M<\/jats:sc>) and 0.1\u2003\u03bc<jats:sc>M<\/jats:sc> ANF increased cyclic GMP content up to 22.9 and 42.3 fold respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In SHR ECs, \u03b1\u2010thrombin\u2010induced calcium transient was not modified by SNAP, ANF or eNOS inhibition. SNAP (100\u2003\u03bc<jats:sc>M<\/jats:sc>) and 0.1\u2003\u03bc<jats:sc>M<\/jats:sc> ANF increased cyclic GMP content up to 9.3 and 51 fold respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In WKY ECs, SNAP dose\u2010dependently (10\u2013100\u2003\u03bc<jats:sc>M<\/jats:sc>) reduced also bradykinin\u2010induced calcium transient, while in SHR ECs was ineffective.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We concluded that in SHR ECs, the cyclic GMP\u2010dependent regulation of calcium transient is lost.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>130<\/jats:bold>, 1468\u20131476; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703453\">10.1038\/sj.bjp.0703453<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703453","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:17:57Z","timestamp":1106947077000},"page":"1468-1476","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Lack of nitric oxide\u2010 and guanosine 3\u2032:5\u2032\u2010cyclic monophosphate\u2010dependent regulation of \u03b1\u2010thrombin\u2010induced calcium transient in endothelial cells of spontaneously hypertensive rat hearts"],"prefix":"10.1111","volume":"130","author":[{"given":"Paola","family":"Failli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Fazzini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"Ruocco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Mazzetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrica","family":"Cecchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Giovannelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Marra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Milani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Giotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.64.6.1127"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(97)00250-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.69.1.107"},{"key":"e_1_2_7_6_1","first-page":"266","article-title":"The cross\u2010talk between nitric oxide and Ca2+: a story with a complex past and a promising future","volume":"18","author":"CLEMENTI E.","year":"1997","journal-title":"TIPS"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.18.4_Suppl.II8"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.2.210"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11023"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1996.0251"},{"key":"e_1_2_7_11_1","first-page":"L954","article-title":"Peroxynitrite modulates receptor\u2010activated Ca2+ signaling in vascular endothelial cells","volume":"270","author":"ELLIOTT S.J.","year":"1996","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.5.C1133"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7079"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.8.4959"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2510243"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)90293-2"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00005211"},{"key":"e_1_2_7_18_1","first-page":"699","article-title":"Cleavage of structural proteins during the assembly of bacteriophage T4","volume":"348","author":"LAEMMLI U.K.","year":"1970","journal-title":"Nature"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70144-6"},{"key":"e_1_2_7_20_1","first-page":"161","volume-title":"Fluorescent and luminescent probes for biological activity. A practical guide to technology for quantitative real\u2010time analysis","author":"MASON W.T.","year":"1993"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1054\/ceca.1999.0034"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.91.9.2310"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-75262-9_11"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3755550"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.23.10480"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)88712-4"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199709)172:3<296::AID-JCP3>3.0.CO;2-J"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90261-V"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199509000-00009"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0895-7061(95)00228-6"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.51.1.10"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99925-4"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0703453","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0703453","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0703453","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T23:36:27Z","timestamp":1745364987000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0703453"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,8]]},"references-count":31,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2000,8]]}},"alternative-id":["10.1038\/sj.bjp.0703453"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0703453","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"type":"print","value":"0007-1188"},{"type":"electronic","value":"1476-5381"}],"subject":[],"published":{"date-parts":[[2000,8]]},"assertion":[{"value":"2009-01-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}