{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:58:45Z","timestamp":1792601925081,"version":"4.5.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3042,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Spontaneously opening, chloride\u2010selective channels that showed outward rectification were recorded in ripped\u2010off patches from rat cultured hippocampal neurons and in cell\u2010attached patches from rat hippocampal CA1 pyramidal neurons in slices.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In both preparations, channels had multiple conductance states and the most common single\u2010channel conductance varied. In the outside\u2010out patches it ranged from 12 to 70\u2003pS (Vp=40\u2003mV) whereas in the cell\u2010attached patches it ranged from 56 to 85\u2003pS (\u2212Vp=80\u2003mV).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Application of GABA to a patch showing spontaneous channel activity evoked a rapid, synchronous activation of channels. During prolonged exposure to either 5 or 100\u2003\u03bc<jats:sc>M<\/jats:sc> GABA, the open probability of channels decreased. Application of GABA appeared to have no immediate effect on single\u2010channel conductance.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Exposure of the patches to 100\u2003\u03bc<jats:sc>M<\/jats:sc> bicuculline caused a gradual decrease on the single\u2010channel conductance of the spontaneous channels. The time for complete inhibition to take place was slower in the outside\u2010out than in the cell\u2010attached patches.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Application of 100\u2003\u03bc<jats:sc>M<\/jats:sc> pentobarbital or 1\u2003\u03bc<jats:sc>M<\/jats:sc> diazepam caused 2\u20134 fold increase in the maximum channel conductance of low conductance (&lt;40\u2003pS) spontaneously active channels.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The observation of spontaneously opening GABA<jats:sub>A<\/jats:sub> channels in cell\u2010attached patches on neurons in slices suggests that they may have a role in neurons <jats:italic>in vivo<\/jats:italic> and could be an important site of action for some drugs such as benzodiazepines, barbiturates and general anaesthetics.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>131<\/jats:bold>, 695\u2013704; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703621\">10.1038\/sj.bjp.0703621<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703621","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:58:37Z","timestamp":1106945917000},"page":"695-704","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Bicuculline, pentobarbital and diazepam modulate spontaneous GABA<sub>A<\/sub> channels in rat hippocampal neurons"],"prefix":"10.1111","volume":"131","author":[{"given":"Bryndis","family":"Birnir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansoureh","family":"Eghbali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea B","family":"Everitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W","family":"Gage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016547"},{"key":"e_1_2_6_3_1","first-page":"291","article-title":"International Union of Pharmacology. 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