{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:05:10Z","timestamp":1760954710130},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3011,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item>\n<jats:p>We have assessed the potential of several \u03bc\u2010opioid receptor antagonists to elicit a response in the guinea\u2010pig isolated ileum in the presence of, and following overnight exposure to, morphine.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Naloxone, <jats:sc>D<\/jats:sc>\u2010Phe\u2010Cys\u2010Tyr\u2010<jats:sc>D<\/jats:sc>\u2010Trp\u2010Orn\u2010Thr\u2010Pen\u2010Thr\u2010NH<jats:sub>2<\/jats:sub> (CTOP), (\u2212)\u20105,9\u03b1\u2010diethyl\u20102\u2010(3\u2010furyl\u2010methyl)\u20102\u2032\u2010hydroxy\u20106,7\u2010benzomorphan (MR2266), but not <jats:sc>D<\/jats:sc>\u2010Phe\u2010Cys\u2010Tyr\u2010<jats:sc>D<\/jats:sc>\u2010Trp\u2010Arg\u2010Thr\u2010Pen\u2010Thr\u2010NH<jats:sub>2<\/jats:sub> (CTAP), produced a transient inhibition of electrically\u2010evoked contractions of the guinea\u2010pig ileum. The effect of 1\u2003\u03bc<jats:sc>M<\/jats:sc> CTOP, but not that to MR2266, was inhibited by 1\u2003\u03bc<jats:sc>M<\/jats:sc> somatostatin.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Naloxone (0.3\u2003\u03bc<jats:sc>M<\/jats:sc>), CTOP (3\u2003\u03bc<jats:sc>M<\/jats:sc>), CTAP (3\u2003\u03bc<jats:sc>M<\/jats:sc>) and MR2266 (0.3\u2003\u03bc<jats:sc>M<\/jats:sc>) antagonized the inhibitory effect of morphine on electrically\u2010evoked contractions of the guinea\u2010pig to a similar degree and, following 60\u2003min exposure to morphine, produced non\u2010sustained contractions. The response to 3\u2003\u03bc<jats:sc>M<\/jats:sc> CTOP was significantly smaller than that to 3\u2003\u03bc<jats:sc>M<\/jats:sc> CTAP. None of the antagonists produced a response in the absence of morphine.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Following overnight exposure of the ileum to 0.3\u2003\u03bc<jats:sc>M<\/jats:sc> morphine (4\u00b0C), and repeated washing to remove the agonist, all four antagonists elicited non\u2010sustained contractions. However, the responses to 3\u2003\u03bc<jats:sc>M<\/jats:sc> CTOP and 0.3\u2003\u03bc<jats:sc>M<\/jats:sc> MR2266 were significantly smaller than those elicited by 0.3\u2003\u03bc<jats:sc>M<\/jats:sc> naloxone and 3\u2003\u03bc<jats:sc>M<\/jats:sc> CTAP. Somatostatin (1\u2003\u03bc<jats:sc>M<\/jats:sc>) significantly reduced naloxone\u2010induced contractions, but not those to CTAP.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>While all four \u03bc\u2010opioid antagonists elicited contractions in the presence of, and following prolonged exposure to, morphine, differences between them were noted which may be a consequence of non\u2010opioid actions.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>131<\/jats:bold>, 893\u2013902; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703659\">10.1038\/sj.bjp.0703659<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703659","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:58:48Z","timestamp":1106945928000},"page":"893-902","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Pharmacological examination of contractile responses of the guinea\u2010pig isolated ileum produced by \u03bc\u2010opioid receptor antagonists in the presence of, and following exposure to, morphine"],"prefix":"10.1111","volume":"131","author":[{"given":"M K","family":"Mundey","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Mason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V G","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"512","article-title":"Alteration in the expression of G\u2010proteins and regulation of adenylate cyclase in human neuroblastoma SH\u2010SY5Y cells chronically exposed to low\u2010efficacy \u03bc\u2010opioids","volume":"280","author":"AMMER H.","year":"1993","journal-title":"Biochem. 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