{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:16:11Z","timestamp":1791270971430,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3011,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item>\n<jats:p>Reduced endothelial nitric oxide (NO) production in conduit vessels for coronary artery bypass grafting (CABG) has been implicated in post\u2010operative complications, including spasm.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>The brief effects of existing NO donors limits their applicability to improving patency of graft vessels. RIG200 is a novel S\u2010nitrosothiol that might have advantages over conventional drugs because it has sustained effects in areas of endothelial damage.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Here we tested the hypothesis that RIG200 and S\u2010nitrosoglutathione (GSNO) have prolonged, NO\u2010mediated effects in human saphenous vein (SV) and internal mammary artery (IMA), compared with glyceryl trinitrate (GTN) and sodium nitroprusside (SNP).<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>84 SV and 80 IMA rings from 64 patients undergoing CABG were studied <jats:italic>in vitro<\/jats:italic>. Rings were precontracted with phenylephrine (EC<jats:sub>80<\/jats:sub> concentration) and the functional integrity of the endothelium tested with acetylcholine (10\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Relaxation of precontracted SV and IMA rings to GTN and SNP (0.01\u201310\u2003\u03bc<jats:sc>M<\/jats:sc>) generally recovered fully on washout. In contrast, responses to RIG200 and GSNO were sustained during washout (30\u2003min). Sustained relaxation was reversed by the NO scavenger, ferrohaemoglobin (10\u2003\u03bc<jats:sc>M<\/jats:sc>) but not by the NO synthase inhibitor, N<jats:sup>\u03c9<\/jats:sup>\u2010nitro\u2010<jats:sc>L<\/jats:sc>\u2010arginine methyl ester (100 and 250\u2003\u03bc<jats:sc>M<\/jats:sc> in SV and IMA respectively).<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Pretreatment (30\u2003min) of SV with both S\u2010nitrosothiols (10\u2003\u03bc<jats:sc>M<\/jats:sc>) inhibited phenylephrine\u2010induced contraction for &gt;180\u2003min, compared with &lt;90\u2003min for GTN. In IMA, contractility was suppressed to 49\u00b14% (GSNO) and 26\u00b14% (RIG200) of baseline after 240\u2003min washout.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Pretreatment of bypass conduits with S\u2010nitrosothiols might improve their patency in the early post\u2010operative period.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>131<\/jats:bold>, 1236\u20131244; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703700\">10.1038\/sj.bjp.0703700<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703700","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:00:40Z","timestamp":1106946040000},"page":"1236-1244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["S\u2010Nitrosothiols cause prolonged, nitric oxide\u2010mediated relaxation in human saphenous vein and internal mammary artery: therapeutic potential in bypass surgery"],"prefix":"10.1111","volume":"131","author":[{"given":"Naoki","family":"Sogo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciro","family":"Campanella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J","family":"Webb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian L","family":"Megson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(98)00206-X"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.eurheartj.a059476"},{"key":"e_1_2_7_4_1","first-page":"249","article-title":"Differential hemodynamic effects and tolerance properties of nitroglycerin and an S\u2010nitrosothiol in experimental heart failure","volume":"256","author":"BAUER J.A","year":"1991","journal-title":"J. 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