{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:46:25Z","timestamp":1773801985162,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2981,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Thrombin causes various cellular events by activating protease\u2010activated receptors (PARs). Here, we showed, for the first time, that thrombin induced myometrial contraction. To determine the mechanism of thrombin\u2010induced myometrial contraction, we simultaneously measured intracellular Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) and tension of fura\u2010PE3\u2010loaded rat myometrium using front\u2010surface fluorimetry. The expression of thrombin receptor mRNA in the rat myometrium were determined by reverse transcription\u2010polymerase chain reaction analysis (RT\u2013PCR analysis).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Thrombin (0.01\u20133\u2003u\u2003ml<jats:sup>\u22121<\/jats:sup>) caused dose\u2010dependent increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and tension in the rat myometrium, and this effect was greatly enhanced in the pregnant myometrium. PAR1\u2010activating peptide mimicked the effects of thrombin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In Ca<jats:sup>2+<\/jats:sup>\u2010free PSS, thrombin induced no increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and tension in the pregnant myometrium. Both diltiazem (10\u2003\u03bc<jats:sc>M<\/jats:sc>) and SK\u2010F 96365 (10\u2003\u03bc<jats:sc>M<\/jats:sc>) significantly inhibited the thrombin\u2010induced elevations of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and tension, and their effects were additive.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>RT\u2013PCR analysis revealed an approximately 10 fold increase in the level of thrombin receptor mRNA in the pregnant myometrium compared to that obtained in the non\u2010pregnant myometrium.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, the contractile response to thrombin was greatly enhanced in the pregnant myometrium, mainly due to the up\u2010regulation of thrombin receptor. We propose that initiation of a post\u2010parturitional myometrial contraction is one of the most important physiological roles of thrombin receptor.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>131<\/jats:bold>, 1619\u20131628; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703729\">10.1038\/sj.bjp.0703729<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703729","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:02:25Z","timestamp":1106946145000},"page":"1619-1628","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Enhanced contractile response to thrombin in the pregnant rat myometrium"],"prefix":"10.1111","volume":"131","author":[{"given":"Yoshinobu","family":"Shintani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuya","family":"Hirano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junji","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoo","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Kanaide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI927"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.28.16466"},{"key":"e_1_2_7_4_1","first-page":"309","article-title":"Inositol, 1,4,5\u2010triphosphate and protein kinase C are involved in thrombin\u2010 and trap\u2010induced vascular smooth muscle contraction","volume":"45","author":"BRETSCHNEIDER E.","year":"1995","journal-title":"Agents Actions (Suppl.)"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.42.24631"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(98)90096-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702303"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(99)01440-6"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70180-0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.29.17349"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/381516a0"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11023"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/1050-1738(94)90013-2"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1992.tb09085.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.6.C1429"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.137.8.3213"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-113-2-742"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb15895.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3130353"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.133.4.1511"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50337-4"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/386502a0"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.11.4152"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(96)70275-2"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(95)00089-4"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(90)91216-Y"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI6042"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/29325"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-250-3:269"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.137.2.780"},{"key":"e_1_2_7_32_1","doi-asserted-by":"crossref","first-page":"3595","DOI":"10.1016\/S0021-9258(19)39631-0","article-title":"Stretching cardiac myocytes stimulates protooncogene expression","volume":"265","author":"KOMURO I.","year":"1990","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.19.12257"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116206"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/35007085"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702004"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.20.9208"},{"key":"e_1_2_7_38_1","first-page":"R541","article-title":"Pregnancy increases ET\u20101\u2010induced contraction and changes receptor subtypes in uterine smooth muscle in humans","volume":"272","author":"OSADA K.","year":"1997","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199811000-00001"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15433.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42184-9"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703410"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1139\/o83-078"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.4.2627"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.133bi.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb14970.x"},{"key":"e_1_2_7_47_1","first-page":"H1962","article-title":"Thrombin receptor\u2010mediated vascular relaxation differentiated by a receptor antagonist and desensitization","volume":"267","author":"TESFAMARIAM B.","year":"1994","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod37.2.348"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.4.723"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90261-V"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.10.6440"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1986.tb34594.x"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.1.C1"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6642"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(92)90631-X"},{"key":"e_1_2_7_56_1","doi-asserted-by":"crossref","first-page":"16975","DOI":"10.1016\/S0021-9258(18)41880-7","article-title":"Molecular cloning of the rat vascular smooth muscle thrombin receptor. Evidence for in vitro regulation by basic fibroblast growth factor","volume":"267","author":"ZHONG C.","year":"1992","journal-title":"J. Biol. Chem."}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0703729","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0703729","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0703729","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T09:16:14Z","timestamp":1698570974000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0703729"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12]]},"references-count":55,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2000,12]]}},"alternative-id":["10.1038\/sj.bjp.0703729"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0703729","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,12]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}