{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T23:35:08Z","timestamp":1772494508539,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2981,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>S\u2010Nitrosothiols are nitric oxide (NO) donor drugs that have been shown to inhibit platelet aggregation in platelet rich plasma (PRP) <jats:italic>in vitro<\/jats:italic> and to inhibit platelet activation <jats:italic>in vivo<\/jats:italic>. The aim of this study was to compare the platelet effects of a novel S\u2010nitrosated glyco\u2010amino acid, RIG200, with an established S\u2010nitrosothiol, S\u2010nitrosoglutathione (GSNO) in PRP, and to investigate the effects of cell\u2010free haemoglobin and red blood cells on S\u2010nitrosothiol\u2010mediated inhibition of platelet aggregation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The effects of GSNO and RIG200 in collagen (2.5\u2003\u03bcg\u2003ml<jats:sup>\u22121<\/jats:sup>)\u2010induced platelet aggregation in PRP and whole blood were investigated <jats:italic>in vitro<\/jats:italic>. Both compounds were found to be powerful inhibitors of aggregation in PRP, and RIG200 was significantly more potent (IC<jats:sub>50<\/jats:sub>=2.0\u2003\u03bc<jats:sc>M<\/jats:sc> for GSNO and 0.8\u2003\u03bc<jats:sc>M<\/jats:sc> for RIG200; <jats:italic>P<\/jats:italic>=0.04).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Neither compound inhibited aggregation in whole blood, even at concentrations of 100\u2003\u03bc<jats:sc>M<\/jats:sc>. Red blood cell concentrations as low as 1% of the haematocrit, and cell\u2010free haemoglobin (2.5\u2003\u03bc<jats:sc>M<\/jats:sc>), significantly reduced their inhibitory effects on platelets.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Experiments involving measurement of cyclic GMP levels, electrochemical detection of NO and electron paramagnetic resonance of haemoglobin in red blood cells, indicated that scavenging of NO generated from S\u2010nitrosothiols by haemoglobin was responsible for the lack of effect of S\u2010nitrosothiols on platelets in whole blood.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These studies suggest that scavenging of NO by haemoglobin in blood might limit the therapeutic application of S\u2010nitrosothiols as anti\u2010platelet agents.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2000) <jats:bold>131<\/jats:bold>, 1391\u20131398; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703731\">10.1038\/sj.bjp.0703731<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703731","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:00:44Z","timestamp":1106946044000},"page":"1391-1398","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Inhibition of human platelet aggregation by a novel S\u2010nitrosothiol is abolished by haemoglobin and red blood cells <i>in vitro<\/i>: implications for anti\u2010thrombotic therapy"],"prefix":"10.1111","volume":"131","author":[{"given":"Ian L","family":"Megson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Sogo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca A","family":"Mazzei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony R","family":"Butler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C","family":"Walton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J","family":"Webb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.8.6.819"},{"key":"e_1_2_7_3_1","first-page":"249","article-title":"Differential hemodynamic effects and tolerance properties of nitroglycerin and an S\u2010nitrosothiol in experimental heart failure","volume":"256","author":"BAUER J.A.","year":"1991","journal-title":"J. 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