{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:23:32Z","timestamp":1773419012334,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2950,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Application of ATP and \u03b1,\u03b2\u2010methylene ATP (\u03b1\u03b2meATP) to voltage\u2010clamped guinea\u2010pig pelvic neurons produced three types of inward currents. A fast\u2010desensitizing response was present in 5% (25\/660) of neurons, 70% gave slowly\u2010desensitizing currents, and the remainder had biphasic responses.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Slowly\u2010desensitizing responses were characterized pharmacologically. The response to \u03b1\u03b2meATP 100\u2003\u03bc<jats:sc>M<\/jats:sc> was 46\u00b127% (range 0\u2003\u2013\u2003100%) of that evoked by ATP 100\u2003\u03bc<jats:sc>M<\/jats:sc> in the same cell. Cross\u2010desensitization indicated the presence of \u03b1\u03b2meATP\u2010sensitive and \u2010insensitive receptors.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The concentration\u2010response curve for \u03b1\u03b2meATP had an EC<jats:sub>50<\/jats:sub> of 55\u2003\u03bc<jats:sc>M<\/jats:sc>, and a Hill coefficient of 0.99, while at the \u03b1\u03b2meATP\u2010insensitive receptor, ATP had an EC<jats:sub>50<\/jats:sub> of 73\u2003\u03bc<jats:sc>M<\/jats:sc>, with a Hill coefficient of 1.78.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The response to \u03b1\u03b2meATP was blocked by pyridoxalphosphate\u20106\u2010azophenyl\u20102\u2032,4\u2032\u2010disulphonic acid (PPADS), suramin and Cibacron blue. However, the \u03b1\u03b2meATP\u2010insensitive receptor was inhibited by PPADS, but not by the other two antagonists.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>2\u2032\u2010 (or 3\u2032\u2010) <jats:italic>O<\/jats:italic>\u2010trinitrophenyl\u2010ATP was 10 times more potent in inhibiting responses to \u03b1\u03b2meATP than to ATP (at the \u03b1\u03b2meATP\u2010insensitive receptor).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Lowering extracellular pH potentiated responses to \u03b1\u03b2meATP and ATP, while raising pH attenuated them.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Co\u2010application of Zn<jats:sup>2+<\/jats:sup> (3\u2003\u2013\u2003300\u2003\u03bc<jats:sc>M<\/jats:sc>) inhibited the responses to \u03b1\u03b2meATP and ATP, with IC<jats:sub>50<\/jats:sub> values of 286 and 60\u2003\u03bc<jats:sc>M<\/jats:sc>, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, unlike rat and mouse pelvic ganglion neurons, which only express P2X<jats:sub>2<\/jats:sub> homomers, at least three distinct P2X receptors are present in guinea\u2010pig pelvic neurons, probably homomeric P2X<jats:sub>2<\/jats:sub>, P2X<jats:sub>3<\/jats:sub> and heteromeric P2X<jats:sub>2\/3<\/jats:sub> receptors. However, some of the novel pharmacological properties observed suggest that the guinea\u2010pig P2X receptor subtypes may differ from their rat orthologues.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>132<\/jats:bold>, 221\u2013233; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703778\">10.1038\/sj.bjp.0703778<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703778","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:06:00Z","timestamp":1106946360000},"page":"221-233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Multiple P2X receptors on guinea\u2010pig pelvic ganglion neurons exhibit novel pharmacological properties"],"prefix":"10.1111","volume":"132","author":[{"given":"Yu","family":"Zhong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip M","family":"Dunn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geoffrey","family":"Burnstock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb16070.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)01203-Q"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/371519a0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00128-2"},{"key":"e_1_2_7_6_1","first-page":"2221","article-title":"P2X receptors: an emerging channel family","volume":"8","author":"BUELL G.","year":"1996","journal-title":"Eur. 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