{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:06:09Z","timestamp":1765231569430},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2950,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effect of two derivatives of salicylate, 2\u2010hydroxy\u20104\u2010trifluoromethylbenzoic acid (HTB) and 2\u2010acetoxy\u20104\u2010trifluoromethylbenzoic acid (triflusal), on the expression of several proteins displaying pro\u2010inflammatory activities the regulation of which is associated to the transcription factor NF\u2010\u03baB, was assayed in the human astrocytoma cell line 1321N1.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) activated NF\u2010\u03baB as judged from both the appearance of \u03baB\u2010binding activity in the nuclear extracts, the degradation of I\u03baB proteins in the cell lysates, and the activation of I\u03baB kinases using an immunocomplex kinase assay with glutathione S\u2010transferase (GST)\u2010I\u03baB proteins as substrates.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>HTB up to 3\u2003m<jats:sc>M<\/jats:sc> did not inhibit the nuclear translocation of NK\u2010\u03baB\/Rel proteins as judged from electrophoretic mobility\u2010shift assays; however, HTB inhibited the degradation of I\u03baB\u03b2 without significantly affecting the degradation of both I\u03baB\u03b1 and I\u03baB\u03b5.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In keeping with their inhibitory effect on I\u03baB\u03b2 degradation in the cell lysates, both HTB and triflusal inhibited the phosphorylation of GST\u2010I\u03baB\u03b2 elicited by TNF\u2010\u03b1, without affecting the phosphorylation of GST\u2010I\u03baB\u03b1.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The effect of both HTB and triflusal on \u03baB\u2010dependent <jats:italic>trans<\/jats:italic>\u2010activation was studied by assaying the expression of both cyclo\u2010oxygenase\u20102 (COX\u20102) and vascular cell adhesion molecule\u20101 (VCAM\u20101). HTB and triflusal inhibited in a dose\u2010dependent manner the expression of COX\u20102 and VCAM\u20101 mRNA and the induction of COX\u20102 protein at therapeutically relevant concentrations.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These findings show the complexity of the biochemical mechanisms underlying the activation of NF\u2010\u03baB in the different cell types and extend the anti\u2010inflammatory effects of HTB and triflusal to neural cells.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>132<\/jats:bold>, 547\u2013555; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703820\">10.1038\/sj.bjp.0703820<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703820","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:09:20Z","timestamp":1106946560000},"page":"547-555","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effect of 4\u2010trifluoromethyl derivatives of salicylate on nuclear factor <i>\u03ba<\/i>B\u2010dependent transcription in human astrocytoma cells"],"prefix":"10.1111","volume":"132","author":[{"given":"Marita","family":"Hern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto Fern\u00e1ndez de","family":"Arriba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manel","family":"Merlos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc\u00eda","family":"Fuentes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariano S\u00e1nchez","family":"Crespo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Luisa","family":"Nieto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.32.22176"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/12.4.547"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3020723"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5234.286"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"887","DOI":"10.4049\/jimmunol.159.2.887","article-title":"Stimulation of Fc\u03b3 receptors in rat peritoneal macrophages induces the expression of nitric oxide synthase and chemokines by mechanisms showing different sensitivities to antioxidants and nitric oxide donors","volume":"159","author":"BAY\u00d3N Y.","year":"1997","journal-title":"J. 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