{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T15:50:07Z","timestamp":1758815407673},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2950,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Although anti\u2010\u03b1<jats:sub>4<\/jats:sub> integrin mAbs reduce eosinophil accumulation in several models of allergic inflammation, it is not clear whether this occurs <jats:italic>via<\/jats:italic> a direct action to block eosinophil \u03b1<jats:sub>4<\/jats:sub> integrins or indirectly on another cell type. The role of \u03b1<jats:sub>4<\/jats:sub> integrins on the accumulation of <jats:sup>111<\/jats:sup>In\u2010labelled eosinophils in allergic and non\u2010allergic inflammation in guinea\u2010pig skin was therefore investigated.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Intradermal injection of antigen in sensitized skin sites induced accumulation of <jats:sup>111<\/jats:sup>In\u2010eosinophils that was reduced up to 70% by two anti\u2010\u03b1<jats:sub>4<\/jats:sub> integrin mAbs. In contrast, accumulation of <jats:sup>111<\/jats:sup>In\u2010eosinophils to intradermal chemoattractants was unaffected by the same mAbs.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Accumulation of <jats:sup>111<\/jats:sup>In\u2010eosinophils in allergic and non\u2010allergic conditions was partly inhibited by a low dose of an anti\u2010\u03b2<jats:sub>2<\/jats:sub> integrin mAb. In combination with anti\u2010\u03b1<jats:sub>4<\/jats:sub> integrin mAb, responses were not further reduced suggesting that these adhesion pathways are not additive or synergic.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Pretreating skin sites with antiserum or contaminating LPS did not reveal an \u03b1<jats:sub>4<\/jats:sub> integrin dependent pathway for chemoattractant\u2010induced <jats:sup>111<\/jats:sup>In\u2010eosinophil accumulation. These data suggest that \u03b1<jats:sub>4<\/jats:sub> integrins are involved in the response to antigen in sensitized skin sites.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Pretreating <jats:sup>111<\/jats:sup>In\u2010eosinophil with \u03b1<jats:sub>4<\/jats:sub> integrin mAb blocked their adhesion to fibronectin <jats:italic>in vitro<\/jats:italic> but did not inhibit their accumulation in allergic inflammation suggesting that the blocking effect <jats:italic>in vivo<\/jats:italic> was eosinophil independent.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data support the concept that targeting \u03b1<jats:sub>4<\/jats:sub> integrins on cells other than eosinophils could control eosinophil accumulation and have therapeutic potential in allergic diseases such as asthma and atopic dermatitis.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>132<\/jats:bold>, 596\u2013604; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0703857\">10.1038\/sj.bjp.0703857<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0703857","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:09:20Z","timestamp":1106946560000},"page":"596-604","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["\u03b1<sub>4<\/sub> integrin\u2010dependent eosinophil recruitment in allergic but not non\u2010allergic inflammation"],"prefix":"10.1111","volume":"132","author":[{"given":"Mauro M","family":"Teixeira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martyn K","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Shock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul G","family":"Hellewell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.3.9609039"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830230925"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.18.5.3052"},{"key":"e_1_2_7_5_1","first-page":"L219","article-title":"Airway recruitment of leukocytes in mice is dependent on alpha4\u2010integrins and vascular cell adhesion molecule\u20101","volume":"272","author":"CHIN J.E.","year":"1997","journal-title":"Am. 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