{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:59:40Z","timestamp":1761764380803},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2830,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,5]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We examined the effect of ischaemia on the neurogenic and nitric oxide (NO)\u2010mediated urethral relaxation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Rabbits were divided into control and urethral ischaemia (UI) groups, which was prepared by the partial occlusion of bilateral iliac arteries using blood vessel occluders.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Neurogenic and NO\u2010mediated proximal urethral relaxation induced by electrical field stimulation (EFS) was greatly impaired in the UI group, while relaxation by sodium nitroprusside (SNP) as a NO donor showed no difference between the two groups. Pretreatment with <jats:sc>L<\/jats:sc>\u2010arginine significantly improved but did not normalize the impaired relaxation in the UI group. Not only basal level, but also stimulated production of cyclic GMP with EFS, were significantly decreased in the UI group.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The tissue contents of N<jats:sup>G<\/jats:sup>\u2010methyl\u2010<jats:sc>L<\/jats:sc>\u2010arginine (<jats:sc>L<\/jats:sc>\u2010NMA) and asymmetric N<jats:sup>G<\/jats:sup>, N<jats:sup>G<\/jats:sup>\u2010dimethyl\u2010<jats:sc>L<\/jats:sc>\u2010arginine (ADMA) in the proximal urethra were increased following ischaemia. While <jats:sc>L<\/jats:sc>\u2010arginine and symmetric N<jats:sup>G<\/jats:sup>, N\u2032<jats:sup>G<\/jats:sup>\u2010dimethyl\u2010<jats:sc>L<\/jats:sc>\u2010arginine (SDMA) contents remained unchanged. Exogenously applied authentic <jats:sc>L<\/jats:sc>\u2010NMA and ADMA (1\u2003\u2013\u2003100\u2003\u03bc<jats:sc>M<\/jats:sc>) concentration\u2010dependently inhibited the EFS\u2010induced urethral relaxation in the control group. The inhibition with <jats:sc>L<\/jats:sc>\u2010NMA and ADMA was undetectable in the presence of 3\u2003m<jats:sc>M L<\/jats:sc>\u2010arginine.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The Ca<jats:sup>2+<\/jats:sup>\u2010dependent NOS activity in the urethra from the UI group was significantly lower than that from the control group and was not restored by an addition of 3\u2003m<jats:sc>M L<\/jats:sc>\u2010arginine.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results suggest that the impaired neurogenic and NO\u2010mediated urethral relaxation with ischaemia is closely related to the increased accumulation of <jats:sc>L<\/jats:sc>\u2010NMA and ADMA and decreased NOS activity, which would result in an accelerated reduction in NO production\/release.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p> <jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>133<\/jats:bold>, 97\u2013106; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704050\">10.1038\/sj.bjp.0704050<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704050","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:24:05Z","timestamp":1106947445000},"page":"97-106","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Involvement of accumulated endogenous NOS inhibitors and decreased NOS activity in the impaired neurogenic relaxation of the rabbit proximal urethra with ischaemia"],"prefix":"10.1111","volume":"133","author":[{"given":"Hitoshi","family":"Masuda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Tsujii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Okuno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazunori","family":"Kihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moritaka","family":"Goto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Azuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)37208-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)62299-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb15910.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1997.0279"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)67391-9"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.278.2.L276"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)51931-X"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.93.1.85"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0870303"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0279"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.95.8.2068"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.3.C750"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.60.070191.003005"},{"key":"e_1_2_6_15_1","first-page":"L194","article-title":"L\u2010arginine restores endothelium\u2010dependent relaxation in pulmonary circulation of chronically hypoxic rats","volume":"263","author":"EDDAHIBI S.","year":"1992","journal-title":"Am. J. Physiol."},{"key":"e_1_2_6_16_1","first-page":"H1522","article-title":"Response of cerebral blood vessels to an endogenous inhibitor of nitric oxide synthase","volume":"269","author":"FARACI F.M.","year":"1995","journal-title":"Am. J. Physiol."},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1998.274.4.L517"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.23.6.1121"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15485.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.30.040190.002535"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.16.5.477"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1992.tb00514.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/117.2.237"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2690207"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.52.167"},{"key":"e_1_2_6_26_1","first-page":"118A","article-title":"Chronic ischemia alters prostate structure and reactivity in the rabbit","volume":"163","author":"KOZLOWSKI R.","year":"2000","journal-title":"J. Urol."},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3430209"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1995.tb00768.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.1930070102"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1991.68"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb13026.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702298"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.16.12.1245.5025"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.50.31213"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13795.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.4.1480"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1982.tb13575.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.2.365"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199204002-00018"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)49711-4"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704050","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704050","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0704050","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T15:00:51Z","timestamp":1697554851000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0704050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,5]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,5]]}},"alternative-id":["10.1038\/sj.bjp.0704050"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0704050","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,5]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}