{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T03:39:27Z","timestamp":1782617967620,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2799,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,6]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The mechanism underlying the LTC<jats:sub>4<\/jats:sub>\u2010induced contraction of guinea\u2010pig taenia coli was determined using the simultaneous measurements of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and force in whole muscle preparations. Additional experiments were performed in receptor coupled permeabilized preparation. For comparison purposes, the contraction which was induced by a typical G\u2010protein mediated agonist, carbachol was also characterized.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>LTC<jats:sub>4<\/jats:sub> induced a contraction in the guinea\u2010pig taenia coli in a concentration\u2010dependent manner. The maximal response was obtained at 100\u2003n<jats:sc>M<\/jats:sc> and the EC<jats:sub>50<\/jats:sub> value was 5.4\u00b11.9\u2003n<jats:sc>M<\/jats:sc>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Both LTC<jats:sub>4<\/jats:sub> and carbachol induced increases in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and force. The maximum force induced by 100\u2003n<jats:sc>M<\/jats:sc> LTC<jats:sub>4<\/jats:sub> was significantly smaller than that induced by 10\u2003\u03bc<jats:sc>M<\/jats:sc> carbachol, although an increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> produced by both agonists was similar. In the permeabilized preparations, carbachol, but not LTC<jats:sub>4<\/jats:sub>, induced an additional force development at a fixed Ca<jats:sup>2+<\/jats:sup> concentration.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>LTC<jats:sub>4<\/jats:sub> induced no increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and force in the Ca<jats:sup>2+<\/jats:sup>\u2010free solution, while carbachol induced transient increases in both [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and force in a Ca<jats:sup>2+<\/jats:sup>\u2010free solution.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Both diltiazem and SK&amp;F 96365 significantly inhibited the LTC<jats:sub>4<\/jats:sub>\u2212 and carbachol\u2010induced increases in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and force in normal PSS. The inhibitory pattern of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> by these drugs was also similar.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We thus conclude that LTC<jats:sub>4<\/jats:sub> induces the contraction of the guinea\u2010pig taenia coli mainly through Ca<jats:sup>2+<\/jats:sup> influx <jats:italic>via<\/jats:italic> both the diltiazem\u2010sensitive and SK&amp;F 96365\u2010sensitive Ca<jats:sup>2+<\/jats:sup> channels, without affecting either the Ca<jats:sup>2+<\/jats:sup>\u2010sensitivity or the intracellular Ca<jats:sup>2+<\/jats:sup> release. These results indicated that the mechanism underlying the LTC<jats:sub>4<\/jats:sub>\u2010induced contraction differs greatly from that for conventional G\u2010protein mediated agonists, such as carbachol.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>133<\/jats:bold>, 529\u2013538; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704122\">10.1038\/sj.bjp.0704122<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704122","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:29:10Z","timestamp":1106947750000},"page":"529-538","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The mechanism for the contraction induced by leukotriene C4 in guinea\u2010pig taenia coli"],"prefix":"10.1111","volume":"133","author":[{"given":"Satoshi","family":"Ieiri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junji","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuya","family":"Hirano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiyo","family":"Suita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideo","family":"Kanaide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.97.24.2406"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-3278(98)90142-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.4049\/jimmunol.137.6.2001","article-title":"Mast cell heterogeneity in higher animals: a comparison of the properties of autologous lung and intestinal mast cells from nonhuman primates","volume":"137","author":"BARRETT K.E.","year":"1986","journal-title":"J. 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