{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:40:57Z","timestamp":1768783257592,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2738,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Immune response\u2010modulating drugs such as thalidomide may be of therapeutic value in the treatment of chronic inflammatory bowel diseases including Crohn's disease (CD). In the present study, we have investigated whether thalidomide exerts this effect by impairing endothelial cell\u2010leukocyte interaction through down\u2010regulation of the expression of pro\u2010inflammatory gene products in these cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Transient CD\u2010like colitis was induced in male Wistar rats by single enema with trinitrobenzene sulphonic acid (TNBS) in ethanol followed by macroscopic scoring, histology, intravital microscopy, RT\u2013PCR and immunohistochemistry (IHC) analyses. Thalidomide or its analogue supidimide were administered in olive oil by intragastric instillation 6\u2003h prior to the induction of colitis and then daily for one week.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Both thalidomide and supidimide (200\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>\u2003d<jats:sup>\u22121<\/jats:sup>) significantly attenuated TNBS\u2010induced colitis as compared to vehicle\u2010treated control animals (44 and 37% inhibition, respectively), and this effect persisted for 7 days post cessation of thalidomide treatment (46% inhibition).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Moreover, thalidomide significantly reduced leukocyte sticking to postcapillary venular endothelial cells in the submucosa (by 45%), improved functional capillary density and perfusion, and attenuated endothelial interleukin\u20108 expression, as judged by IHC analysis. According to RT\u2013PCR analysis, both thalidomide and supidimide also significantly reduced vascular cell adhesion molecule\u20101 mRNA expression in the affected part of the descending colon.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These findings suggest that thalidomide and one of its derivatives impairs CD\u2010like TNBS\u2010induced colitis in the rat by down\u2010regulating endothelial adhesion molecule and chemokine expression and, as a consequence, the interaction of these cells with circulating leukocytes.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>133<\/jats:bold>, 1414\u20131423; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704193\">10.1038\/sj.bjp.0704193<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704193","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:58:51Z","timestamp":1106949531000},"page":"1414-1423","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Thalidomide impairment of trinitrobenzene sulphonic acid\u2010induced colitis in the rat\u2013role of endothelial cell\u2010leukocyte interaction"],"prefix":"10.1111","volume":"133","author":[{"given":"Bianca","family":"Lienenl\u00fcke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomislav","family":"Stojanovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Fiebig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afshin","family":"Fayyazi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tieno","family":"Germann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Hecker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.38.6.911"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1999.01538.x"},{"key":"e_1_2_7_4_1","first-page":"1101","article-title":"The metabolic fate of supidimide in the rat","volume":"32","author":"BECKER R.","year":"1982","journal-title":"Arzneimittelforsch."},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70638-9"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.163.1.380"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"4211","DOI":"10.4049\/jimmunol.147.12.4211","article-title":"Differential effects of neutrophil\u2010activating peptide 1\/IL\u20108 and its homologues on leukocyte adhesion and phagocytosis","volume":"147","author":"DETMERS P.A.","year":"1991","journal-title":"J. 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