{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:30:16Z","timestamp":1759336216113},"reference-count":57,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2738,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Calcitonin gene\u2010related peptide (CGRP), amylin and adrenomedullin (AM) belong to the same family of peptides. Accumulating evidence indicate that the calcitonin (CT) receptor, the CT receptor\u2010like receptor (CRLR) and receptor\u2010activity\u2010modifying proteins (RAMPs) form the basis of all the receptors in this family of peptides.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Using reverse transcriptase\u2013polymerase chain reaction the presence of mRNA sequences encoding the CRLR, RAMP1 and RAMP2 were demonstrated in porcine left anterior descending (LAD) coronary arteries, whereas porcine calcitonin (CT) receptor mRNA was not present. The partial porcine mRNA sequences shared 82\u201392% nucleotide identity with human sequences.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The human peptides \u03b1CGRP, \u03b2CGRP, AM and amylin induced relaxation with pEC<jats:sub>50<\/jats:sub> values of 8.1, 8.1, 6.7 and 6.1\u2003<jats:sc>M<\/jats:sc> respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The antagonistic properties of a novel non\u2010peptide CGRP antagonist \u2018Compound 1\u2019 (WO98\/11128), \u03b2CGRP<jats:sub>8\u201337<\/jats:sub> and the proposed AM receptor antagonist AM<jats:sub>22\u201352<\/jats:sub> were compared to the well\u2010known CGRP<jats:sub>1<\/jats:sub> receptor antagonist \u03b1CGRP<jats:sub>8\u201337<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The \u03b1CGRP<jats:sub>8\u201337<\/jats:sub> and \u03b2CGRP<jats:sub>8\u201337<\/jats:sub> induced concentration\u2010dependent (10<jats:sup>\u22127<\/jats:sup>\u201310<jats:sup>\u22125<\/jats:sup>\u2003<jats:sc>M<\/jats:sc>) rightward shift of both the \u03b1CGRP and \u03b2CGRP concentration\u2010response curves. \u03b2CGRP<jats:sub>8\u201337<\/jats:sub> (10<jats:sup>\u22126<\/jats:sup>\u2003<jats:sc>M<\/jats:sc>) had the same effect as \u03b1CGRP<jats:sub>8\u201337<\/jats:sub> (10<jats:sup>\u22126<\/jats:sup>\u2003<jats:sc>M<\/jats:sc>), but with less potent rightward shift of the concentration\u2010response curves for \u03b1CGRP, AM and amylin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Preincubation with \u2018Compound 1\u2019 (10<jats:sup>\u22127<\/jats:sup>\u201310<jats:sup>\u22125<\/jats:sup>\u2003<jats:sc>M<\/jats:sc>) and AM<jats:sub>22\u201352<\/jats:sub> (10<jats:sup>\u22126<\/jats:sup>\u2003<jats:sc>M<\/jats:sc>) had no significant antagonistic effect.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, the building blocks forming CGRP and AM receptors were present in the porcine LAD, whereas those of the amylin receptor were not. \u03b1CGRP, \u03b2CGRP, AM and amylin mediated vasorelaxation <jats:italic>via<\/jats:italic> the CGRP receptors. No functional response was detected to adrenomedullin <jats:italic>via<\/jats:italic> the adrenomedullin receptor.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>133<\/jats:bold>, 1405\u20131413; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704210\">10.1038\/sj.bjp.0704210<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704210","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:58:51Z","timestamp":1106949531000},"page":"1405-1413","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["CGRP receptors mediating CGRP\u2010, adrenomedullin\u2010 and amylin\u2010induced relaxation in porcine coronary arteries. 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