{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:32:52Z","timestamp":1761237172230},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2711,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The adenosine receptor in mouse pinealocytes was identified and characterized using pharmacological and physiological approaches.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Expression of the two adenosine receptor subtypes A<jats:sub>2B<\/jats:sub> and A<jats:sub>3<\/jats:sub> was detected in mouse pineal glands and PGT\u2010\u03b2 cells by polymerase chain reaction and nucleotide sequencing.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Adenosine and 5\u2032\u2010N\u2010ethylcarboxamidoadenosine (NECA) evoked cyclic AMP generation but the A<jats:sub>2A<\/jats:sub>\u2010selective agonist 2\u2010(4\u2010(2\u2010carboxyethyl)phenylethylamino)adenosine\u20105\u2032\u2010N\u2010ethylcarboxamideadenosine (CGS 21680) and the A<jats:sub>1<\/jats:sub>\u2010specific agonists R\u2010N<jats:sup>6<\/jats:sup>\u2010(2\u2010phenylisopropyl)adenosine (R\u2010PIA) and N<jats:sup>6<\/jats:sup>\u2010cyclopentyladenosine (CPA) had little effect on intracellular cyclic AMP levels. The A<jats:sub>2B<\/jats:sub> receptor selective antagonists alloxazine and enprofylline completely blocked NECA\u2010mediated cyclic AMP accumulation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Treatment of cells with the A<jats:sub>3<\/jats:sub>\u2010selective agonist N<jats:sup>6<\/jats:sup>\u2010(3\u2010iodobenzyl)\u20105\u2032\u2010(N\u2010methylcarbamoyl)adenosine (IB\u2010MECA) inhibited the elevation of the cyclic AMP level induced by NECA or isoproterenol in a concentration\u2010dependent manner with maximal inhibition of 40\u2003\u2013\u200350%. These responses were blocked by the specific A<jats:sub>3<\/jats:sub> adenosine receptor antagonist MRS 1191. Pretreatment of the cells with pertussis toxin attenuated the IB\u2010MECA\u2010induced responses, suggesting that this effect occurred <jats:italic>via<\/jats:italic> the pertussis toxin\u2010sensitive inhibitory G proteins.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>IB\u2010MECA also caused a concentration\u2010dependent elevation in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and IP<jats:sub>3<\/jats:sub> content. Both the responses induced by IB\u2010MECA were attenuated by treatment with U73122 or phorbol 12\u2010myristate 13\u2010acetate.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data suggest the presence of both A<jats:sub>2B<\/jats:sub> and A<jats:sub>3<\/jats:sub> adenosine receptors in mouse pineal tumour cells and that the A<jats:sub>2B<\/jats:sub> receptor is positively coupled to adenylyl cyclase whereas the A<jats:sub>3<\/jats:sub> receptor is negatively coupled to adenylyl cyclase and also coupled to phospholipase C.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>134<\/jats:bold>, 132\u2013142; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704218\">10.1038\/sj.bjp.0704218<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704218","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:59:14Z","timestamp":1106949554000},"page":"132-142","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Pharmacological characterization of adenosine receptors in PGT\u2010\u03b2 mouse pineal gland tumour cells"],"prefix":"10.1111","volume":"134","author":[{"given":"Byung\u2010Chang","family":"Suh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae\u2010Don","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung\u2010Uek","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Je\u2010Kyung","family":"Seong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyong\u2010Tai","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.52.5.846"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(94)90879-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1128(200003)20:2<103::AID-MED1>3.0.CO;2-X"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(05)80056-X"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(94)90480-4"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1210561"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2650421"},{"key":"e_1_2_7_9_1","first-page":"532","article-title":"A reagent for the single\u2010step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples","volume":"15","author":"CHOMCZYNSKI P.","year":"1993","journal-title":"Biotechniques"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-0186(00)00034-6"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2560959"},{"key":"e_1_2_7_12_1","first-page":"125","article-title":"Neural pathways and neurotransmitters affecting melatonin synthesis","volume":"21","author":"EBADI M.","year":"1986","journal-title":"J. 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