{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:28:34Z","timestamp":1773419314638,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2711,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>In myometrial strips from near\u2010term non\u2010labouring human uterus, addition of oxytocin (OT) evoked dose\u2010dependent (10\u2003\u2013\u20033000\u2003n<jats:sc>M<\/jats:sc>) phasic contractions that were antagonized by atosiban (1\u2003\u03bc<jats:sc>M<\/jats:sc>) and relaxed by addition of the nitric oxide donor S\u2010nitroso L\u2010cysteine (Cys\u2010NO). In near\u2010term labouring myometrium, however, addition of OT was ineffective at raising additional tone.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In both labouring and non\u2010labouring tissue, Cys\u2010NO mediated relaxation of spontaneous or OT\u2010induced contractions (IC<jats:sub>50<\/jats:sub>=1\u2003\u03bc<jats:sc>M<\/jats:sc>) was unaffected by prior addition of the guanylyl cyclase (GC) inhibitors ODQ (1H\u2010[1,2,4]oxadiazolo[4,3,\u2010\u03b1]quinoxalin\u20101\u2010one; 1\u2003\u03bc<jats:sc>M<\/jats:sc>), or methylene blue (MB; 10\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Elevation of intracellular cyclic GMP accompanying 30\u2003\u03bc<jats:sc>M<\/jats:sc> Cys\u2010NO addition in non\u2010labouring tissue (7.5 fold) or in labouring tissues (2.5 fold) was completely blocked in tissues that had been pre\u2010treated with ODQ or MB.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Charybdotoxin (ChTx), iberiotoxin (IbTx) and kaliotoxin (KalTx) all shifted the Cys\u2010NO inhibition curve to the right and reduced the degree of relaxation produced by maximal Cys\u2010NO treatment (100\u2003\u03bc<jats:sc>M<\/jats:sc> in non\u2010labouring tissue; in labouring tissue, KalTx prevented Cys\u2010NO mediated relaxation in both stimulated and unstimulated tissue.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Addition of the NO\u2010donor S\u2010nitroso N\u2010acetyl penicillamine (SNAP) produced a dose\u2010dependent relaxation of pregnant myometrium while 3\u2010morpholinosyndonimine (SIN\u20101) did not. The failure of SIN\u20101 to relax OT\u2010induced contractions was not due to a failure of the donor to stimulate myometrial GC.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We demonstrate that despite the ability of NO to stimulate myometrial GC in pregnant uterine muscle, relaxations are independent of cyclic GMP action. Effects of K<jats:sup>+<\/jats:sup>\u2010channel inhibitors suggests that NO\u2010induced relaxation in human uterine smooth muscle may be subserved by direct or indirect activation of one or more calcium\u2010activated K<jats:sup>+<\/jats:sup>\u2010channels.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>134<\/jats:bold>, 206\u2013214; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704226\">10.1038\/sj.bjp.0704226<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704226","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:59:14Z","timestamp":1106949554000},"page":"206-214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["NO\u2010induced relaxation of labouring and non\u2010labouring human myometrium is not mediated by cyclic GMP"],"prefix":"10.1111","volume":"134","author":[{"given":"Iain L O","family":"Buxton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A","family":"Kaiser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas A","family":"Malmquist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Tichenor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.00165.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/368850a0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.6.C1668"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BFb0048263"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(95)90500-6"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90343-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)44447-4"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00044067-200005000-00010"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1997.272.6.H2765"},{"key":"e_1_2_7_11_1","first-page":"422","article-title":"Lack of correlation between cyclic GMP elevation and relaxation of nonvascular smooth muscle by nitroglycerin, nitroprusside, hydroxylamine and sodium azide","volume":"225","author":"DIAMOND J.","year":"1983","journal-title":"J. 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