{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T02:28:25Z","timestamp":1759026505919},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2711,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:p><jats:list list-type=\"explicit-label\"><jats:list-item><jats:p>This study was undertaken to investigate the possible contribution of the blockade of eotaxin generation to the anti\u2010eosinophilotactic effect of phosphodiesterase (PDE) type 4 inhibitors. In some experiments, the putative synergistic interaction between PDE type 4 inhibitors and the \u03b22\u2010agonist salbutamol was also assessed.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Sensitized guinea\u2010pigs aerosolized with antigen (5% ovalbumin, OVA) responded with a significant increase in eotaxin and eosinophil levels in the bronchoalveolar lavage fluid (BALF) at 6\u2003h. Eosinophil recruitment was inhibited by both PDE type 4 inhibitors rolipram (5\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>, i.p.) and RP 73401 (5\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>, i.p.) treatments. In contrast, only rolipram inhibited eotaxin production.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Sensitized rats intrapleurally challenged (i.pl.) with antigen (OVA, 12\u2003\u03bcg\u2003cavity<jats:sup>\u22121<\/jats:sup>) showed a marked eosinophil infiltration at 24\u2003h, preceded by eotaxin generation at 6\u2003h. Intravenous administration of a rabbit anti\u2010mouse eotaxin antibody (0.5\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>) significantly reduced allergen\u2010evoked eosinophilia in this model.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Local pretreatment with rolipram (40\u2003\u03bcg\u2003cavity<jats:sup>\u22121<\/jats:sup>) or RP 73401 (40\u2003\u03bcg\u2003cavity<jats:sup>\u22121<\/jats:sup>) 1\u2003h before challenge reduced eosinophil accumulation evaluated in the rat pleural effluent, but only the former was active against eotaxin generation. The inhibitors of PDE type 3 (SK&amp;F 94836) and type 5 (zaprinast) failed to alter allergen\u2010evoked eosinophil recruitment in rats.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Local injection of \u03b22\u2010agonist salbutamol (20\u2003\u03bcg\u2003cavity<jats:sup>\u22121<\/jats:sup>) inhibited both eosinophil accumulation and eotaxin production following pleurisy. The former was better inhibited when salbutamol and rolipram were administered in combination.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Treatment with rolipram and RP 73401 dose\u2010dependently inhibited eosinophil adhesion and migration<jats:italic>in vitro<\/jats:italic>. These effects were clearly potentiated by salbutamol at concentrations that had no effect alone.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Our findings indicate that although rolipram and RP 73401 are equally effective in inhibiting allergen\u2010induced eosinophil infiltration only the former prevents eotaxin formation, indicating that PDE 4 inhibitors impair eosinophil accumulation by mechanisms independent of eotaxin production blockade.<\/jats:p><\/jats:list-item><\/jats:list><\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic>(2001)<jats:bold>134<\/jats:bold>, 283\u2013294; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704233\">10.1038\/sj.bjp.0704233<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704233","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T22:01:54Z","timestamp":1106949714000},"page":"283-294","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Modulation of eotaxin formation and eosinophil migration by selective inhibitors of phosphodiesterase type 4 isoenzyme"],"prefix":"10.1111","volume":"134","author":[{"given":"Patr\u00edcia M R","family":"Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra C","family":"Alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magda F","family":"Serra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Lucia A","family":"Pires","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliane P","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emiliano O","family":"Barreto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato S B","family":"Cordeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J","family":"Jose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro M","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Lagente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco A","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(96)00357-3"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.2.1029"},{"key":"e_1_2_7_4_1","first-page":"674","article-title":"The ability of phosphodiesterase IV inhibitors to supress superoxide production in guinea pig eosinophils is correlated with inhibition of phosphodiesterase IV catalytic activity","volume":"273","author":"BARNETTE M.S.","year":"1995","journal-title":"J. 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