{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:42:46Z","timestamp":1773290566998,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2677,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effects of agents that inactivate free radical nitric oxide (carboxy\u2010PTIO, hydroxocobalamin and pyrogallol) were tested on relaxations produced by the nitroxyl anion (NO<jats:sup>\u2212<\/jats:sup>) donor Angeli's salt in rat aortic rings and anococcygeus muscles. The amount of NO<jats:sup>\u2022<\/jats:sup> generated from Angeli's salt in the presence of these agents was measured using a NO<jats:sup>\u2022<\/jats:sup>\u2010selective electrode sensor.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Carboxy\u2010PTIO (100, 300\u2003\u03bc<jats:sc>M<\/jats:sc>), hydroxocobalamin (30, 100\u2003\u03bc<jats:sc>M<\/jats:sc>) and pyrogallol (10, 30\u2003\u03bc<jats:sc>M<\/jats:sc>) significantly reduced relaxations produced by Angeli's salt (0.3\u2003\u03bc<jats:sc>M<\/jats:sc>) in aortic rings but not in anococcygeus muscles.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>NO<jats:sup>\u2022<\/jats:sup> generated from Angeli's salt (0.1\u2003\u2013\u200310\u2003\u03bc<jats:sc>M<\/jats:sc>), as detected by the sensor electrode, was less than 0.5% of the amount of Angeli's salt added. Carboxy\u2010PTIO (100\u2003\u03bc<jats:sc>M<\/jats:sc>) and hydroxocobalamin (30\u2003\u03bc<jats:sc>M<\/jats:sc>), but not pyrogallol significantly increased the amount of NO<jats:sup>\u2022<\/jats:sup> detected.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the presence of an oxidizing agent copper [II] (as CuSO<jats:sub>4<\/jats:sub> 100\u2003\u03bc<jats:sc>M<\/jats:sc>), the amount of NO<jats:sup>\u2022<\/jats:sup> detected from 0.3\u2003\u03bc<jats:sc>M<\/jats:sc> of Angeli's salt increased from an undetectable level of 142.7\u00b115.7\u2003n<jats:sc>M<\/jats:sc> (equivalent to 47.6% of Angeli's salt added). Under these conditions, carboxy\u2010PTIO, hydroxocobalamin and pyrogallol significantly reduced the amount of NO<jats:sup>\u2022<\/jats:sup> detected from Angeli's salt as well as the signal generated by an equivalent amount of authentic NO (0.33\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The difference in effects of these agents on relaxations to Angeli's salt in the aorta and the anococcygeus muscle may be explained by the ready conversion of NO<jats:sup>\u2212<\/jats:sup> to NO<jats:sup>\u2022<\/jats:sup> in the aorta through an unidentified mechanism, which makes NO<jats:sup>\u2212<\/jats:sup> susceptible to inactivation by these agents. Furthermore, in addition to inactivating NO<jats:sup>\u2022<\/jats:sup>, carboxy\u2010PTIO and hydroxocobalamin may themselves oxidize NO<jats:sup>\u2212<\/jats:sup> to NO<jats:sup>\u2022<\/jats:sup>, albeit slightly.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>134<\/jats:bold>, 521\u2013528; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704287\">10.1038\/sj.bjp.0704287<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704287","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T22:03:41Z","timestamp":1106949821000},"page":"521-528","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Effects of agents that inactivate free radical NO (NO<sup>\u2022<\/sup>) on nitroxyl anion\u2010mediated relaxations, and on the detection of NO<sup>\u2022<\/sup> released from the nitroxyl anion donor Angeli's salt"],"prefix":"10.1111","volume":"134","author":[{"given":"Anthie","family":"Ellis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun Guang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J","family":"Rand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004337200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00054a013"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1995.1231"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(92)90315-U"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.4.1620"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1182\/blood.V88.5.1857.1857","article-title":"Nitric oxide interactions with cobalamins: biochemical and functional consequences","volume":"88","author":"BROUWER M.","year":"1996","journal-title":"Blood"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703812"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(96)00078-0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00210a047"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00510-X"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703058"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(92)90584-6"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb14677.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/34402"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/niox.1998.0170"},{"key":"e_1_2_7_17_1","volume-title":"Free Radicals in Biology and Medicine","author":"HALLIWELL B.","year":"1999"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12483.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(99)00019-5"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(97)01381-2"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01152a005"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(00)00156-8"},{"key":"e_1_2_7_23_1","first-page":"665","article-title":"Spectroscopic studies of nitric oxide (NO) interactions with cobalamins: reactions of NO with superoxocabalamin(III) likely accounts for cobalamin reversal of the biological effects of NO","volume":"285","author":"KRUSZYNA H.","year":"1998","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15264.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702277"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702540"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1989.tb02404.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701571"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm00115a013"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03714.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.23.10860"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703550"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00845-1"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15333.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.1668"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13429.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(93)90210-9"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb16681.x"},{"key":"e_1_2_7_39_1","first-page":"48","article-title":"Interactions between hydroxocobalamin and nitric oxide (NO): evidence for a redox reaction between NO and reduced cobalamin and reversible NO binding to oxidised cobalamin","volume":"275","author":"ROCHELLE L.G.","year":"1995","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.25.14492"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90269-0"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)90028-0"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3300719"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi973153g"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704287","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704287","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0704287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T12:19:15Z","timestamp":1697545155000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0704287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,10]]}},"alternative-id":["10.1038\/sj.bjp.0704287"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0704287","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}