{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:33:09Z","timestamp":1774564389494,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2646,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We examined the effect of SP100030, a novel inhibitor of activator protein\u20101 (AP\u20101) and nuclear factor (NF)\u2010\u03baB transcription factors, in a rat model of asthma.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Sensitized Brown\u2010Norway rats were treated with SP100030 (20\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>\u2003day<jats:sup>\u22121<\/jats:sup> for 3 days) intraperitoneally prior to allergen challenge. Allergen exposure of sensitized rats induced bronchial hyperresponsiveness (BHR), accumulation of inflammatory cells in bronchoalveolar lavage (BAL) fluid, and also an increase in eosinophils and CD2<jats:sup>+<\/jats:sup>, CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup>\u2003T\u2010cells in the airways together with mRNA expression for IL\u20102, IL\u20104, IL\u20105, IL\u201010, and IFN\u2010\u03b3.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Pre\u2010treatment with SP100030 inhibited BAL lymphocyte influx (<jats:italic>P<\/jats:italic>&lt;0.03), specifically reduced CD8<jats:sup>+<\/jats:sup> T\u2010cell infiltration in the airway submucosa (<jats:italic>P<\/jats:italic>&lt;0.03), and mRNA expression for IL\u20102, IL\u20105, and IL\u201010 (<jats:italic>P<\/jats:italic>&lt;0.05). Neutrophil, eosinophil, and CD4<jats:sup>+<\/jats:sup>\u2003T\u2010cells accumulation in the airways and BHR were not affected by SP100030.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Our results indicate that suppression of IL\u20102 and IL\u20105 mRNA expression may not necessarily lead to suppression of BHR. The expression of IL\u20105 mRNA may contribute to the airway accumulation of eosinophils, but does not correlate with the extent of eosinophilia.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The joint AP\u20101 and NF\u2010\u03baB inhibitor, SP100030, selectively inhibits CD8<jats:sup>+<\/jats:sup> T\u2010cells, and mRNA expression of both Th1 and Th2 cytokines <jats:italic>in vivo<\/jats:italic>, but does not inhibit allergen\u2010induced airway eosinophilia and BHR.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>134<\/jats:bold>, 1029\u20131036; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704344\">10.1038\/sj.bjp.0704344<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704344","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:34:12Z","timestamp":1106948052000},"page":"1029-1036","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["A novel transcription factor inhibitor, SP100030, inhibits cytokine gene expression, but not airway eosinophilia or hyperresponsiveness in sensitized and allergen\u2010exposed rat"],"prefix":"10.1111","volume":"134","author":[{"given":"Tung\u2010Jung","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian M","family":"Adcock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K Fan","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"129","article-title":"The role of Jun, Fos and the AP\u20101 complex in cell\u2010proliferation and transformation","volume":"1072","author":"ANGEL P.","year":"1991","journal-title":"Biochem. Biophy. Acta."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/142.6_Pt_1.1407"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1995.tb00421.x"},{"key":"e_1_2_7_5_1","first-page":"515","article-title":"Inflammatory mediators of asthma: an update","volume":"50","author":"BARNES P.J.","year":"1998","journal-title":"Pharm. Rev."},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"4412","DOI":"10.4049\/jimmunol.155.9.4412","article-title":"Inhibition of macrophage inflammatory protein\u20101 alpha expression by IL\u201010. Differential sensitivities in human blood monocytes and alveolar macrophages","volume":"155","author":"BERKMAN N.","year":"1995","journal-title":"J. Immunol."},{"key":"e_1_2_7_7_1","first-page":"257","article-title":"Normal bronchial epithelial cells constitutively produce the antiinflammatory cytokine interleukin\u201010 which is down\u2010regulated in cystic fibrosis","volume":"13","author":"BONFIELD T.L.","year":"1995","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1994.tb00920.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.2207"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.41.9.657"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.54.9.825"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/141.4_Pt_1."},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.1.109"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/7.2.128"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(91)90253-K"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.4.561"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.146.10.3444"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.1.195"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.3.1652"},{"key":"e_1_2_7_21_1","first-page":"3106","article-title":"SP100030 is a novel T\u2010cell\u2010specific transcription factor inhibitor that possesses immunosuppressive activity in vivo","volume":"28","author":"GOLDMAN M.E.","year":"1996","journal-title":"Transplant. Proc."},{"key":"e_1_2_7_22_1","first-page":"591","article-title":"T\u2010cells subsets and activation in bronchial mucosa of sensitized Brown\u2010Norway rats after single allergen exposure","volume":"85","author":"HACZKU A.","year":"1995","journal-title":"Immunology"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.155.3.9117011"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115166"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5155"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.158.5.9706116"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.20.5.3473"},{"key":"e_1_2_7_28_1","doi-asserted-by":"crossref","first-page":"5054","DOI":"10.4049\/jimmunol.161.9.5054","article-title":"Prevention of Th2\u2010like cell responses by coadministration of IL\u201012 and IL\u201018 is associated with inhibition of antigen\u2010induced airway hyperresponsiveness, eosinophilia, and serum IgE levels","volume":"161","author":"HOFSTRA C.L.","year":"1998","journal-title":"J. Immunol."},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1999.00699.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1999.00870.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70337-4"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000237196"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(96)70096-9"},{"key":"e_1_2_7_34_1","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.4049\/jimmunol.160.3.1378","article-title":"Induction of pulmonary allergic responses by antigen\u2010specific Th2 cells","volume":"160","author":"LI X.M.","year":"1998","journal-title":"J. Immunol."},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.152.2.7633694"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/148.6_Pt_1.1623"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.3.889"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1994.tb39811.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00711.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(96)00492-7"},{"issue":"7","key":"e_1_2_7_41_1","first-page":"48","article-title":"Inflammation and the late phase reaction in asthma: the effect of polymorphonuclear leukocyte depletion on airways obstruction and bronchial hyperreactivity in an animal model","volume":"22","author":"MURPHY K.R.","year":"1986","journal-title":"Bull. Eur. Physiopathol. Respir."},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1164\/arrd.1984.130.2.214"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116725"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199201303260504"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V79.12.3101.bloodjournal79123101"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.10.110194.002201"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3131876"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.2.711"},{"key":"e_1_2_7_49_1","first-page":"A760","article-title":"Inhibitory effects of IL\u201010 on allergen\u2010induced airway inflammation and airway responses in Brown\u2010Norway rats","volume":"149","author":"WOOLLEY M.J.","year":"1994","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.9.1739"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117966"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704344","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704344","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0704344","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T13:34:28Z","timestamp":1697549668000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0704344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11]]},"references-count":50,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2001,11]]}},"alternative-id":["10.1038\/sj.bjp.0704344"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0704344","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,11]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}