{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:49:43Z","timestamp":1761540583403},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2646,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The pharmacological characteristics of solid\u2010phase von Willebrand factor (svWF), a novel platelet agonist, were studied.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Washed platelet suspensions were obtained from human blood and the effects of svWF on platelets were measured using aggregometry, phase\u2010contrast microscopy, flow cytometry and zymography.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Incubation of platelets with svWF (0.2\u2003\u2013\u20031.2\u2003\u03bcg\u2003ml<jats:sup>\u22121<\/jats:sup>) resulted in their adhesion to the ligand, while co\u2010incubations of svWF with subthreshold concentrations of ADP, collagen and thrombin resulted in aggregation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>6B4 inhibitory anti\u2010glycoprotein (GP)Ib antibodies abolished platelet adhesion stimulated by svWF, while aggregation was reduced in the presence of 6B4 and N\u2010Acetyl\u2010Pen\u2010Arg\u2010Gly\u2010Asp\u2010Cys, an antagonist of GPIIb\/IIIa.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Platelet adhesion stimulated with svWF was associated with a concentration\u2010dependent increase in expression of GPIb, but not of GPIIb\/IIIa.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In contrast, collagen (0.5\u2003\u2013\u200310.0\u2003\u03bcg\u2003ml<jats:sup>\u22121<\/jats:sup>) caused down\u2010regulation of GPIb and up\u2010regulation of GPIIb\/IIIa in platelets.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Solid\u2010phase vWF (1.2\u2003\u03bcg\u2003ml<jats:sup>\u22121<\/jats:sup>) resulted in the release of MMP\u20102 from platelets.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Inhibition of MMP\u20102 with phenanthroline (10\u2003\u03bc<jats:sc>M<\/jats:sc>), but not with aspirin or apyrase, inhibited platelet adhesion stimulated with svWF.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In contrast, human recombinant MMP\u20102 potentiated both the effects of svWF on adhesion and up\u2010regulation of GPIb.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Platelet adhesion and aggregation stimulated with svWF were reduced by S\u2010nitroso\u2010n\u2010acetyl\u2010penicillamine, an NO donor, and prostacyclin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Thus, stimulation of human platelets with svWF leads to adhesion and aggregation that are mediated <jats:italic>via<\/jats:italic> activation of GPIb and GPIIb\/IIIa, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Mechanisms of activation of GPIb by svWF involve the release of MMP\u20102, and are regulated by NO and prostacyclin.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>134<\/jats:bold>, 1013\u20131020; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704345\">10.1038\/sj.bjp.0704345<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704345","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:34:12Z","timestamp":1106948052000},"page":"1013-1020","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Pharmacological characteristics of solid\u2010phase von Willebrand factor in human platelets"],"prefix":"10.1111","volume":"134","author":[{"given":"Anna","family":"Radomski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael W","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Jurasz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek W","family":"Radomski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"3217","article-title":"Sur on nouvelle vari\u00e9t\u00e9 de dystrophie thrombocytaire h\u00e9morragipare cong\u00e9nitale","volume":"24","author":"BERNARD J.","year":"1948","journal-title":"Sem. 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