{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T15:39:03Z","timestamp":1762443543518},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2616,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>In some asthmatics, muscarinic receptor antagonists are effective in limiting bronchoconstrictor response, suggesting an abnormal cholinergic drive in these subjects. There is a growing body of evidences indicating that cholinergic neurotransmission is also enhanced by endothelin\u20101 (ET\u20101) in rabbit bronchi, mouse trachea and in human isolated airway preparations.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We investigated the role of secondary mediators in ET\u20101 induced potentiation of cholinergic nerve\u2010mediated contraction in human bronchi, in particular the possible role of neuropeptides in this phenomenon.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Bronchial tissues after endothelin treatment were exposed to a standard electrical field stimulation (EFS) (30% of EFS 30Hz)\u2010induced contraction. In addition, in some experiments, preparations were treated with a tachykinin NK<jats:sub>2<\/jats:sub> receptor antagonist and subsequently exposed to the same protocol. HPLC and RIA were performed on organ bath fluid samples. Moreover, the human bronchi were used for the \u03b2\u2010PPT (preprotachykinin) mRNA extraction and semiquantitative reverse transcription polymerase chain reaction (RT\u2003\u2013\u2003PCR), prior to and 30\u2003\u2013\u200340\u2003min following ET\u20101 challenge.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The selective tachykinin NK<jats:sub>2<\/jats:sub> receptor antagonist, SR48968, was effective to reduce ET\u20101 potentiation of EFS mediated contraction. HPLC or RIA showed significant increased quantities of NKA in organ bath effluents after EFS stimulation in bronchi pretreated with ET\u20101. Finally, \u03b2\u2010PPT mRNA level after stimulation of bronchi with ET\u20101 was increased about 2 fold respect to control untreated bronchi.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, this study demonstrated that, at least in part, the ET\u20101 potentiation of cholinergic nerve\u2010mediated contraction is mediated by tachykinin release, suggesting that in addition to nerves, several type of cells, such as airway smooth muscle cell, may participate to neuropeptide production.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2001) <jats:bold>134<\/jats:bold>, 1447\u20131454; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704395\">10.1038\/sj.bjp.0704395<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704395","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:36:10Z","timestamp":1106948170000},"page":"1447-1454","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Endothelin\u20101 increases cholinergic nerve\u2010mediated contraction of human bronchi <b><i>via<\/i><\/b> tachykinin synthesis induction"],"prefix":"10.1111","volume":"134","author":[{"given":"Bruno","family":"D'Agostino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Advenier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maddalena","family":"Falciani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Gallelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppina","family":"Marrocco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Piegari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelia","family":"Filippelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Rossi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000195867"},{"key":"e_1_2_7_3_1","first-page":"1289","article-title":"Neuronal control of human airways in health and disease","volume":"134","author":"BARNES P.J.","year":"1986","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90777-4"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1992.72.3.699"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2125.1996.03721.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb14844.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/139.3.806"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(99)00051-X"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-07-02203.1990"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.2000.15e26.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(85)90012-3"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/140.2.449"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00005293"},{"key":"e_1_2_7_15_1","first-page":"L648","article-title":"Cytokine\u2010 and virus\u2010stimulated airway smooth muscle cells produce IL\u201011 and other IL\u20106\u2010type cytokines","volume":"273","author":"ELIAS J.A.","year":"1997","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.149.1.8111568"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(92)90352-P"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.1999.14b33.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15617.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12509.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4652(200002)186:2<307::AID-JCP1029>3.0.CO;2-I"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.1999.14d08.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.159.6.9805039"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.9.5257"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119122"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703866"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb17176.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15465.x"},{"key":"e_1_2_7_29_1","first-page":"1","volume-title":"Asthma: Basic Mechanism and Clinical Management","author":"HOGG J.C.","year":"1988"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(88)90064-4"},{"key":"e_1_2_7_31_1","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.4049\/jimmunol.158.4.1841","article-title":"Human airway smooth muscle cells express and release RANTES in response to T helper 1 cytokines: regulation by T helper 2 cytokines and corticosteroids","volume":"158","author":"JOHN M.","year":"1997","journal-title":"J. Immunol."},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(86)80282-0"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.3.881"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.3.807"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.16.1.8998077"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0742-8413(89)90109-6"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199322008-00049"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/306032a0"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/312729a0"},{"key":"e_1_2_7_40_1","article-title":"Secretion of nerve growth factor (NGF) from human lung fibroblasts in culture effect of inflammatory cytokines","volume":"336","author":"OLGART C.","year":"1999","journal-title":"Am. J. Respir. Crit. Care. Med"},{"key":"e_1_2_7_41_1","first-page":"551","article-title":"Receptors and antagonists for substance P and related peptides","volume":"46","author":"REGOLI D.","year":"1994","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.94.07122226"},{"key":"e_1_2_7_43_1","first-page":"13","article-title":"The innervation of the lung","volume":"117","author":"RICHARDSON J.B.","year":"1982","journal-title":"Eur. J. Respir. Dis. Suppl."},{"key":"e_1_2_7_44_1","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1152\/jappl.1988.64.5.1979","article-title":"Thromboxane effects on canine trachealis neuromuscular function","volume":"64","author":"SERIO R.","year":"1988","journal-title":"J. Appl. Physiol."},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1989.tb08551.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1152\/jappl.1982.53.1.169","article-title":"Dose\u2010dependent inhibition of cold air\u2010induced bronchoconstriction by atropine","volume":"53","author":"SHEPPARD D.","year":"1982","journal-title":"J. Appl. Physiol."},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.158.supplement_2.13tac300"},{"key":"e_1_2_7_48_1","first-page":"1073","article-title":"The effect of sensory nerve depletion on cholinergic neurotransmission in guinea\u2010pig airways","volume":"260","author":"STRETTON C.D.","year":"1992","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)92497-2"},{"key":"e_1_2_7_50_1","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1152\/jappl.1986.60.2.458","article-title":"Effect of substance P on neurally mediated contraction of rabbit airway smooth muscle","volume":"60","author":"TANAKA D.T.","year":"1986","journal-title":"J. Appl. Physiol."},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.95.08010127"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(91)90175-P"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.282.6264.598"},{"key":"e_1_2_7_54_1","first-page":"1084","article-title":"Endothelin ETA and ETB receptors facilitating parasympathetic neurotransmission in the rabbit trachea","volume":"275","author":"YONEYAMA T.","year":"1995","journal-title":"J. Pharmacol. Exp. Ther."}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704395","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704395","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0704395","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:47:44Z","timestamp":1697417264000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0704395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12]]},"references-count":53,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1038\/sj.bjp.0704395"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0704395","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,12]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}