{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T09:15:45Z","timestamp":1773047745908,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2558,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Hypertension development, phenylephrine\u2010induced contraction and Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>\u2010ATPase functional activity and protein expression in aorta (AO), tail (TA) and superior mesenteric (SMA) arteries from ouabain\u2010 (25\u2003\u03bcg day<jats:sup>\u22121<\/jats:sup>, s.c., 5 weeks) and vehicle\u2010treated rats were evaluated.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Ouabain treatment increased systolic blood pressure (127\u00b11 <jats:italic>vs<\/jats:italic> 160\u00b12\u2003mmHg, <jats:italic>n<\/jats:italic>=24, 35; <jats:italic>P<\/jats:italic>&lt;0.001) while the maximum response to phenylephrine was reduced (<jats:italic>P<\/jats:italic>&lt;0.01) in AO (102.8\u00b13.9 <jats:italic>vs<\/jats:italic> 67.1\u00b110.1% of KCl response, <jats:italic>n<\/jats:italic>=12, 9) and SMA (82.5\u00b17.5 <jats:italic>vs<\/jats:italic> 52.2\u00b15.8%, <jats:italic>n<\/jats:italic>=12, 9).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Endothelium removal potentiated the phenylephrine response to a greater extent in segments from ouabain\u2010treated rats. Thus, differences of area under the concentration\u2010response curves (dAUC) in endothelium\u2010denuded and intact segments for control and ouabain\u2010treated rats were, respectively: AO, 56.6\u00b19.6 <jats:italic>vs<\/jats:italic> 198.3\u00b118.3 (<jats:italic>n<\/jats:italic>=9, 7); SMA, 85.5\u00b115.4 <jats:italic>vs<\/jats:italic> 165.4\u00b124.8 (<jats:italic>n<\/jats:italic>=6, 6); TA, 13.0\u00b16.1 <jats:italic>vs<\/jats:italic> 39.5\u00b110.4% of the corresponding control AUC (<jats:italic>n<\/jats:italic>=6, 6); <jats:italic>P<\/jats:italic>&lt;0.05.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The relaxation to KCl (1\u2003\u2013\u200310\u2003m<jats:sc>M<\/jats:sc>) was similar in segments from both groups. Compared to controls, the inhibition of 0.1\u2003m<jats:sc>M<\/jats:sc> ouabain on KCl relaxation was greater in AO (dAUC: 64.8\u00b14.6 <jats:italic>vs<\/jats:italic> 84.0\u00b15.1%, <jats:italic>n<\/jats:italic>=11, 14; <jats:italic>P<\/jats:italic>&lt;0.05), similar in SMA (dAUC: 39.1\u00b13.9 <jats:italic>vs<\/jats:italic> 43.3\u00b17.8%, <jats:italic>n<\/jats:italic>=6, 7; <jats:italic>P<\/jats:italic>&gt;0.05) and smaller in TA (dAUC: 62.1\u00b15.5 <jats:italic>vs<\/jats:italic> 41.4\u00b18.2%, <jats:italic>n<\/jats:italic>=12, 13; <jats:italic>P<\/jats:italic>&lt;0.05) in ouabain\u2010treated rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Protein expression of both \u03b1<jats:sub>1<\/jats:sub> and \u03b1<jats:sub>2<\/jats:sub> isoforms of Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>\u2010ATPase was augmented in AO, unmodified in SMA and reduced in TA from ouabain\u2010treated rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results suggest that chronic administration of ouabain induces hypertension and regional vascular alterations, the latter possibly as a consequence of the hypertension.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p> <jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>135<\/jats:bold>, 771\u2013781; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704501\">10.1038\/sj.bjp.0704501<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704501","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:43:10Z","timestamp":1106948590000},"page":"771-781","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Alterations in phenylephrine\u2010induced contractions and the vascular expression of Na<sup>+<\/sup>,K<sup>+<\/sup>\u2010ATPase in ouabain\u2010induced hypertension"],"prefix":"10.1111","volume":"135","author":[{"given":"Luciana V","family":"Rossoni","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mercedes","family":"Salaices","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Mar\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalton V","family":"Vassallo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda J","family":"Alonso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"520","article-title":"Norepinephrine\u2010induced relaxations in rat aorta mediated by endothelial beta adrenoceptors. Impairment by ageing and hypertension","volume":"270","author":"ARRIBAS S.","year":"1994","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.275.5.F633"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.6.C1367"},{"key":"e_1_2_7_5_1","first-page":"307","volume-title":"Endocrine Mechanisms in Hypertension.","author":"BUCKALEW V.M.","year":"1989"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200004000-00004"},{"key":"e_1_2_7_7_1","first-page":"682","article-title":"Enhancement of alpha\u20101 and alpha\u20102 adrenergic agonist\u2010induced vasoconstriction by removal of endothelium in rat aorta","volume":"232","author":"CARRIER G.O.","year":"1985","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_8_1","first-page":"S52","article-title":"Effects of norepinephrine on 86Rb\u2010uptake by arteries from spontaneously hypertensive and Wistar\u2010Kyoto rats","volume":"4","author":"COX R.M.","year":"1986","journal-title":"J. Hypertens."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00679-8"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.28.5.732"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.86.2.420"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199602000-00002"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/300650a0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2838907"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.23.6.1087"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.28.6.1005"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.34.1.107"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.23.Supplement_S67"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1995.tb02327.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00278-7"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.19.6.721"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.23.Supplement_S77"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199405000-00008"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(99)00037-6"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(97)00051-X"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1988.tb11403.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.17.6.930"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-198704000-00006"},{"key":"e_1_2_7_29_1","first-page":"239","article-title":"Variation in sensitivity of alpha adrenoceptor\u2010mediated contraction of the vascular smooth muscle of rabbit elastic and muscular arteries is related to receptor affinity","volume":"241","author":"ORIOWO M.A.","year":"1987","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.38.6.48"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-200018070-00009"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1996.270.3.E386"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1159\/000159145"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.26.1.177"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.20.5.674"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199912000-00017"},{"key":"e_1_2_7_37_1","first-page":"163","article-title":"Regulation of Na+,K+\u2010ATPase \u03b1\u2010subunit isoforms in rat tissues during hypertension","volume":"292","author":"SAHIN\u2010ERDEMLI I.","year":"1995","journal-title":"Eur. J. Pharmacol."},{"key":"e_1_2_7_38_1","first-page":"H350","article-title":"Rat vascular tissues express all three \u03b1\u2010isoforms of Na+,K+\u2010ATPase","volume":"266","author":"SAHIN\u2010ERDEMLI I.","year":"1994","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.27.3.827"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(89)90019-1"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15255.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X1997000400016"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.276.1.H63"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.23.Supplement_S55"},{"key":"e_1_2_7_45_1","first-page":"198","article-title":"Potassium\u2010induced relaxations as an indicator of Na+,K+\u2010ATPase activity in vascular smooth muscle","volume":"15","author":"WEBB R.C.","year":"1978","journal-title":"Blood Vessels"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00657652"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-199303000-00005"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/28.7.957"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.22.2.178"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704501","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0704501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T01:56:37Z","timestamp":1697507797000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0704501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2]]},"references-count":48,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,2]]}},"alternative-id":["10.1038\/sj.bjp.0704501"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0704501","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2]]},"assertion":[{"value":"2009-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}