{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:04:24Z","timestamp":1773281064271,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2558,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We investigated subtypes of alpha\u20101 adrenoceptor (AR) in rabbit ocular tissues using reverse transcription\u2010polymerase chain reaction (RT\u2003\u2013\u2003PCR), <jats:italic>in situ<\/jats:italic> hybridization (ISH) and binding studies.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Competitive RT\u2003\u2013\u2003PCR assays specific for the subtypes of alpha\u20101 AR revealed that the mRNA expression of alpha\u20101a AR was dominant, and that of each alpha\u20101b and alpha\u20101d was less than 10% and 0.5% of total alpha\u20101 ARs mRNA, respectively, in the iris, ciliary body, choroid and retina.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In alpha\u20101a AR splice isoform\u2010specific RT\u2003\u2013\u2003PCR assays, we found a distinct proportion of each isoform mRNA in the iris, ciliary body and choroid.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results of the ISH assays for alpha\u20101a AR subtype showed that hybridization signals were clearly observed in the iris dilator muscle and in the epithelium of the ciliary processes.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In binding studies, alpha\u20101A AR was a dominant subtype in the iris, choroid and retina in contrast to the ciliary body that had more alpha\u20101B than alpha\u20101A AR subtype at protein level.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p> <jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>135<\/jats:bold>, 600\u2013608; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704503\">10.1038\/sj.bjp.0704503<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704503","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:43:10Z","timestamp":1106948590000},"page":"600-608","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Distribution of alpha\u20101 adrenoceptor subtypes in RNA and protein in rabbit eyes"],"prefix":"10.1111","volume":"135","author":[{"given":"Fumiko","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takanobu","family":"Taniguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seigo","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshio","family":"Akagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikara","family":"Kubota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikunobu","family":"Muramatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_3_1","first-page":"121","article-title":"International union of pharmacology nomenclature of adrenoceptors","volume":"46","author":"BYLUND D.B.","year":"1994","journal-title":"Pharmacol. 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