{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:52:12Z","timestamp":1790509932847,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2585,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:p>Cannabinoids have an antinociceptive action in many pain models. We have investigated a possible modulatory role for Type 1 Cannabinoid receptors (CB<jats:sub>1<\/jats:sub>) on the release of excitatory transmitter Substance P from the adult mouse spinal cord after stimulation of nociceptor terminals by capsaicin. Capsaicin (0.1\u2003\u2013\u200310\u2003\u03bc<jats:sc>M<\/jats:sc>) was applied to superfused cord sections and evoked a dose dependent release of SP above basal outflow of (23.36\u00b12.96\u2003fmol\u20038\u2003ml<jats:sup>\u22121<\/jats:sup>). Maximum evoked SP release was obtained with 5\u2003\u03bc<jats:sc>M<\/jats:sc> Capsaicin (262.4\u00b120.8\u2003fmol\u20038\u2003ml<jats:sup>\u22121<\/jats:sup>). Higher capsaicin concentrations (50\u2003\u2013\u2003100\u2003\u03bc<jats:sc>M<\/jats:sc>) evoked less SP release. Superfusion of CB<jats:sub>1<\/jats:sub> antagonist SR141716A (5\u2003\u03bc<jats:sc>M<\/jats:sc>) increased evoked SP release with capsaicin (0.1\u2003\u2013\u200310\u2003\u03bc<jats:sc>M<\/jats:sc>) and reversed the reducing effect of high dose capsaicin (100\u2003\u03bc<jats:sc>M<\/jats:sc>). Antagonism of CB<jats:sub>1<\/jats:sub> receptors in the spinal cord during capsaicin stimulation, is evidence of tonic CB<jats:sub>1<\/jats:sub> activity inhibiting the release of excitatory transmitters after activation of nociceptive neurones and is also indicative of endocannabinoid production during noxious stimulation.<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>135<\/jats:bold>, 21\u201324; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704506\">10.1038\/sj.bjp.0704506<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704506","type":"journal-article","created":{"date-parts":[[2006,6,7]],"date-time":"2006-06-07T19:20:37Z","timestamp":1149708037000},"page":"21-24","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["CB<sub>1<\/sub> receptor antagonist SR141716A increases capsaicin\u2010evoked release of Substance P from the adult mouse spinal cord"],"prefix":"10.1111","volume":"135","author":[{"given":"I J","family":"Lever","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Malcangio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(00)00389-4"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(90)90237-3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/39807"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0752434.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1038\/372686a0","article-title":"Formation and inactivation of endogenous cannabinoid anandamide in central neurons","volume":"372","author":"DI MARZO V.","year":"1993","journal-title":"Nature"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(01)01012-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2000.0844"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0954-6820.2000.00773.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00534-5"},{"key":"e_1_2_7_11_1","first-page":"143","article-title":"Capsaicin: cellular targets mechanisms of action and selectivity for thin sensory neurons","volume":"43","author":"HOLZER P.","year":"1991","journal-title":"Pharmacol. 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