{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:38:02Z","timestamp":1772894282524,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2530,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The flavonol quercetin has been shown to activate a Cl<jats:sup>\u2212<\/jats:sup> secretion in rat colon. Unlike the secretory activity of the related isoflavone genistein, quercetin's secretory activity does not depend on cyclic AMP; instead, it depends on Ca<jats:sup>2+<\/jats:sup>. We investigated the possible involvement of Ca<jats:sup>2+<\/jats:sup> dependent basolateral K<jats:sup>+<\/jats:sup> channels using apically permeabilized rat distal colon epithelium mounted in Ussing chambers.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In intact epithelium, quercetin induced an increase in short\u2010circuit current (<jats:italic>I<\/jats:italic><jats:sub>sc<\/jats:sub>), which was diminished by the Cl<jats:sup>\u2212<\/jats:sup> channel blockers NPPB and DPC, but not by glibenclamide, DIDS or anthracene\u20109\u2010carboxylic acid. The effect of the flavonol was also inhibited by several serosally applied K<jats:sup>+<\/jats:sup> channel blockers (Ba<jats:sup>2+<\/jats:sup>, quinine, clotrimazole, tetrapentylammonium, 293B), whereas other K<jats:sup>+<\/jats:sup> channel blockers failed to influence the quercetin\u2010induced increase in <jats:italic>I<\/jats:italic><jats:sub>sc<\/jats:sub> (tetraethylammonium, charybdotoxin).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The apical membrane was permeabilized by mucosal addition of nystatin and a serosally directed K<jats:sup>+<\/jats:sup> gradient was applied. The successful permeabilization was confirmed by experiments demonstrating the failure of bumetanide to inhibit the carbachol\u2010induced current.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In apically permeabilized epithelium, quercetin induced a K<jats:sup>+<\/jats:sup> current (<jats:italic>I<\/jats:italic><jats:sub>K<\/jats:sub>), which was neither influenced by ouabain nor by bumetanide. Whereas DPC, NPPB, charybdotoxin and 293B failed to inhibit this <jats:italic>I<\/jats:italic><jats:sub>K<\/jats:sub>, quinine, Ba<jats:sup>2+<\/jats:sup>, clotrimazole and tetrapentylammonium were effective blockers of this current.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We conclude from these results that at least part of the quercetin\u2010induced Cl<jats:sup>\u2212<\/jats:sup> secretion can be explained by an activation of basolateral K<jats:sup>+<\/jats:sup> channels.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>135<\/jats:bold>, 1183\u20131190; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704564\">10.1038\/sj.bjp.0704564<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704564","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:46:17Z","timestamp":1106948777000},"page":"1183-1190","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The flavonol quercetin activates basolateral K<sup>+<\/sup> channels in rat distal colon epithelium"],"prefix":"10.1111","volume":"135","author":[{"given":"Rainer","family":"Cermak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisela","family":"Kuhn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siegfried","family":"Wolffram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002100100410"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900372"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050229"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.2.C619"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1998.275.5.G1166"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-445X.2000.01923.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.92.3.281"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.2.479"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15563.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00375048"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240051125"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.62.1.467"},{"key":"e_1_2_7_14_1","first-page":"62","article-title":"Regulation of ion transport in colonic crypts","volume":"12","author":"GREGER R.","year":"1997","journal-title":"News Physiol. 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