{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:28:26Z","timestamp":1791163706240,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2530,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:p>\n                    <jats:list list-type=\"explicit-label\">\n                      <jats:list-item>\n                        <jats:p>The aim of this study was to provide a pharmacological characterization of the Y receptor types responsible for neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP) effects upon electrogenic ion transport in isolated human colonic mucosa.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Preparations of descending colon were voltage\u2010clamped at 0\u2003mV in Ussing chambers and changes in short\u2010circuit current (I\n                          <jats:sub>sc<\/jats:sub>\n                          ) continuously recorded. Basolateral PYY, NPY, human PP (hPP), PYY(3\u2003\u2013\u200336), [Leu\n                          <jats:sup>31<\/jats:sup>\n                          , Pro\n                          <jats:sup>34<\/jats:sup>\n                          ]PYY (Pro\n                          <jats:sup>34<\/jats:sup>\n                          PYY) and [Leu\n                          <jats:sup>31<\/jats:sup>\n                          , Pro\n                          <jats:sup>34<\/jats:sup>\n                          ]\u2010NPY (Pro\n                          <jats:sup>34<\/jats:sup>\n                          NPY) all reduced basal I\n                          <jats:sub>sc<\/jats:sub>\n                          in untreated colon. Of all the Y agonists tested PYY(3\u2003\u2013\u200336) responses were most sensitive to tetrodotoxin (TTX) pretreatment, indicating that Y\n                          <jats:sub>2<\/jats:sub>\n                          \u2010receptors are located on intrinsic neurones as well as epithelia in this tissue.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          The EC\n                          <jats:sub>50<\/jats:sub>\n                          values for Pro\n                          <jats:sup>34<\/jats:sup>\n                          PYY, PYY(3\u2003\u2013\u200336) and hPP were 9.7\u2003n\n                          <jats:sc>M<\/jats:sc>\n                          (4.0\u2003\u2013\u200323.5), 11.4\u2003n\n                          <jats:sc>M<\/jats:sc>\n                          (7.6\u2003\u2013\u200317.0) and 14.5\u2003n\n                          <jats:sc>M<\/jats:sc>\n                          (10.2\u2003\u2013\u200320.5) and response curves exhibited similar efficacies. The novel Y\n                          <jats:sub>5<\/jats:sub>\n                          agonist [Ala\n                          <jats:sup>31<\/jats:sup>\n                          , Aib\n                          <jats:sup>32<\/jats:sup>\n                          ]\u2010NPY had no effect at 100\u2003n\n                          <jats:sc>M<\/jats:sc>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Y\n                          <jats:sub>1<\/jats:sub>\n                          receptor antagonists, BIBP3226 and BIBO3304 both increased basal I\n                          <jats:sub>sc<\/jats:sub>\n                          levels\n                          <jats:italic>per se<\/jats:italic>\n                          and inhibited subsequent PYY and Pro\n                          <jats:sup>34<\/jats:sup>\n                          PYY but not hPP or PYY(3\u2003\u2013\u200336) responses. The Y\n                          <jats:sub>2<\/jats:sub>\n                          antagonist, BIIE0246 also raised basal I\n                          <jats:sub>sc<\/jats:sub>\n                          levels and attenuated subsequent PYY(3\u2003\u2013\u200336) but not Pro\n                          <jats:sup>34<\/jats:sup>\n                          PYY or hPP responses.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We conclude that Y\n                          <jats:sub>1<\/jats:sub>\n                          and Y\n                          <jats:sub>2<\/jats:sub>\n                          receptor\u2010mediated inhibitory tone exists in human colon mucosa. PYY and NPY exert their effects via both Y\n                          <jats:sub>1<\/jats:sub>\n                          and Y\n                          <jats:sub>2<\/jats:sub>\n                          receptors, but the insensitivity of hPP responses to either Y\n                          <jats:sub>1<\/jats:sub>\n                          or Y\n                          <jats:sub>2<\/jats:sub>\n                          antagonism, or to TTX, indicates that Y\n                          <jats:sub>4<\/jats:sub>\n                          receptors are involved and that they are predominantly post\u2010junctional in human colon.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>British Journal of Pharmacology<\/jats:italic>\n                    (2001)\n                    <jats:bold>135<\/jats:bold>\n                    , 1505\u20131512; doi:\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704604\">10.1038\/sj.bjp.0704604<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1038\/sj.bjp.0704604","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T16:48:57Z","timestamp":1106930937000},"page":"1505-1512","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Neuropeptide Y, Y\n                    <sub>1<\/sub>\n                    , Y\n                    <sub>2<\/sub>\n                    and Y\n                    <sub>4<\/sub>\n                    receptors mediate Y agonist responses in isolated human colon mucosa"],"prefix":"10.1111","volume":"135","author":[{"given":"Helen M","family":"Cox","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain R","family":"Tough","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.45.26762"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(84)90034-X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000626200"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000007496"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb12695.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017029"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(91)90020-P"},{"key":"e_1_2_7_9_1","first-page":"102P","article-title":"Responses to Y receptor agonists are insensitive to haloperidol pretreatment in human and mouse colonic mucosae in vitro","volume":"128","author":"COX H.M.","year":"1999","journal-title":"Br. 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