{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:58:15Z","timestamp":1791917895761,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2530,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item>\n<jats:p>The role of internal Ca<jats:sup>2+<\/jats:sup> stores in the generation of the rhythmic electrical and contractile activity in the guinea\u2010pig proximal renal pelvis was examined using intracellular microelectrode and muscle tension recording techniques.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Ryanodine (30\u2003\u03bc<jats:sc>M<\/jats:sc>) transiently increased contraction amplitude, while caffeine (0.5\u2003\u2013\u20033\u2003m<jats:sc>M<\/jats:sc>) reduced contraction amplitude and frequency. Contractility was also reduced by 2\u2010aminoethoxy\u2010diphenylborate (2\u2010APB 60\u2003\u03bc<jats:sc>M<\/jats:sc>), xestospongin C (1\u2003\u03bc<jats:sc>M<\/jats:sc>), U73122 (5\u2003\u03bc<jats:sc>M<\/jats:sc>) and neomycin (4\u2003m<jats:sc>M<\/jats:sc>), blockers of IP<jats:sub>3<\/jats:sub>\u2010dependent release from Ca<jats:sup>2+<\/jats:sup> stores.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>60\u2003m<jats:sc>M<\/jats:sc> K<jats:sup>+<\/jats:sup> saline\u2010evoked contractions were reduced by caffeine (1\u2003m<jats:sc>M<\/jats:sc>), U73122 (5\u2003\u03bc<jats:sc>M<\/jats:sc>) and neomycin (4\u2003m<jats:sc>M<\/jats:sc>), but little affected by ryanodine or 2\u2010APB (60\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Spontaneous action potentials consisting of an initial spike followed by a long plateau were recorded (frequency 8.6\u00b11.0\u2003min<jats:sup>\u22121<\/jats:sup>) in small urothelium\u2010denuded strips of proximal renal pelvis.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Action potential discharge was blocked in 75 and 35% of cells by 2\u2010APB (60\u2003\u03bc<jats:sc>M<\/jats:sc>) and caffeine (1\u2003m<jats:sc>M<\/jats:sc>), respectively. In the remaining cells, only a truncation of the plateau phase was observed.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Cyclopiazonic acid (CPA 10\u2003\u03bc<jats:sc>M<\/jats:sc> for 10\u2003\u2013\u2003180\u2003min), blocker of CaATPase, transiently increased contraction frequency and amplitude. Action potential durations were increased 3.6 fold. Contraction amplitude and frequency slowly declined during a prolonged (&gt;60\u2003min) CPA exposure.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>We conclude that the action potential in caffeine\u2010sensitive cells and the shoulder component of caffeine\u2010insensitive action potential arise from the entry of Ca<jats:sup>2+<\/jats:sup> through Ca<jats:sup>2+<\/jats:sup> channels. The inhibitory actions of modulators of internal Ca<jats:sup>2+<\/jats:sup> release were partially explained by a blockade of Ca<jats:sup>2+<\/jats:sup> entry.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>135<\/jats:bold>, 1363\u20131374; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704609\">10.1038\/sj.bjp.0704609<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704609","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:48:57Z","timestamp":1106948937000},"page":"1363-1374","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Modulators of internal Ca<sup>2+<\/sup> stores and the spontaneous electrical and contractile activity of the guinea\u2010pig renal pelvis"],"prefix":"10.1111","volume":"135","author":[{"given":"R J","family":"Lang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Hashitani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Keller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Takano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E L","family":"Mulholland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Fukuta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0855s.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703104"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0827h.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.515ae.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002400050159"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006605"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80384-0"},{"key":"e_1_2_7_9_1","first-page":"418","article-title":"Species variation in the location of upper urinary tract pacemaker cells","volume":"11","author":"GOSLING J.A.","year":"1974","journal-title":"Invest. 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