{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T16:22:53Z","timestamp":1771950173116,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2499,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item>\n<jats:p>The effects of ascorbate were assessed on vasodilatation mediated by endothelium\u2010derived hyperpolarizing factor (EDHF) in the ciliary vascular bed of the bovine isolated perfused eye and in the rat isolated perfused mesenteric arterial bed.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>In the bovine eye, EDHF\u2010mediated vasodilator responses induced by acetylcholine or bradykinin were powerfully blocked when ascorbate (50\u2003\u03bc<jats:sc>M<\/jats:sc>) was included in the perfusion medium for at least 120\u2003min; with acetylcholine a normally\u2010masked muscarinic vasoconstrictor response was also uncovered.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>The blockade of EDHF\u2010mediated vasodilatation by ascorbate was time\u2010dependent (maximum blockade at 120\u2003min) and concentration\u2010dependent (10\u2003\u2013\u2003150\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Ascorbate (50\u2003\u03bc<jats:sc>M<\/jats:sc>) also blocked acetylcholine\u2010induced, EDHF\u2010mediated vasodilator responses in the rat mesenteric arterial bed in a time\u2010dependent manner (maximum blockade at 180\u2003min).<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>The ability of ascorbate to block EDHF\u2010mediated vasodilatation is likely to result from its reducing properties, since this action was mimicked in the bovine eye by two other reducing agents, namely, N\u2010acetyl\u2010<jats:sc>L<\/jats:sc>\u2010cysteine (1\u2003m<jats:sc>M<\/jats:sc>) and dithiothreitol (100\u2003\u03bc<jats:sc>M<\/jats:sc>), but not by the redox\u2010inactive analogue, dehydroascorbate (50\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>In conclusion, concentrations of ascorbate present in normal plasma block EDHF\u2010mediated vasodilator responses in the bovine eye and rat mesentery. The mechanism and physiological consequences of this blockade remain to be determined.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>135<\/jats:bold>, 1801\u20131809; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704623\">10.1038\/sj.bjp.0704623<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704623","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T21:50:25Z","timestamp":1106949025000},"page":"1801-1809","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Ascorbate blocks endothelium\u2010derived hyperpolarizing factor (EDHF)\u2010mediated vasodilatation in the bovine ciliary vascular bed and rat mesentery"],"prefix":"10.1111","volume":"135","author":[{"given":"Alister J","family":"McNeish","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William S","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199303000-00011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nu.11.070191.001041"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb17172.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.94.12.3341"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900252"},{"key":"e_1_2_7_7_1","first-page":"H2413","article-title":"Prologue: EDHF \u2010 what is it?","volume":"280","author":"CAMPBELL W.B.","year":"2001","journal-title":"Am. 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