{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T20:32:37Z","timestamp":1774125157493,"version":"3.50.1"},"reference-count":67,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2408,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Sepsis induced by <jats:italic>S. aureus<\/jats:italic> was used to investigate whether neutrophil migration failure to infectious focus correlates with lethality in Gram\u2010positive bacteria\u2010induced sepsis in mice.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>By contrast with the sub\u2010lethal (SL\u2010group), the lethal (L\u2010group) intraperitoneal inoculum of <jats:italic>S. aureus<\/jats:italic> caused failure of neutrophil migration (92% reduction), high CFU in the exudate, bacteremia and impairment of <jats:italic>in vitro<\/jats:italic> neutrophil chemotactic activity.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Pre\u2010treatments of L\u2010group with adequate doses of aminoguanidine prevented the neutrophil migration failure and improved the survival of the animals (pre\u2010treated: 43%; untreated: 0% survival). Thus, the impairment of neutrophil migration in the L\u2010group appears to be mediated by nitric oxide (NO).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The injection of <jats:italic>S. aureus<\/jats:italic> SL\u2010inoculum in iNOS deficient (\u2212\/\u2212) or aminoguanidine\u2010treated wild\u2010type mice (pre\u2010 and post\u2010treatment), which did not present neutrophil migration failure, paradoxically caused severe peritonitis and high mortality. This fact is explainable by the lack of NO dependent microbicidal activity in migrated neutrophils.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, although the NO microbicidal mechanism is active in neutrophils, the failure of their migration to the infectious focus may be responsible for the severity and outcome of sepsis.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>136<\/jats:bold>, 645\u2013658; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704734\">10.1038\/sj.bjp.0704734<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704734","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T22:12:23Z","timestamp":1106950343000},"page":"645-658","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Involvement of NO in the failure of neutrophil migration in sepsis induced by <i>Staphylococcus aureus<\/i>"],"prefix":"10.1111","volume":"136","author":[{"given":"D P","family":"Crosara\u2010Alberto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A L C","family":"Darini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R Y","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J S","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S H","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F Q","family":"Cunha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1998.00644.x"},{"key":"e_1_2_7_3_1","first-page":"331","article-title":"Evaluation of factors affecting mortality rate after sepsis in a murine caecal ligation and puncture model","volume":"94","author":"BAKER C.C.","year":"1983","journal-title":"Surgery"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(05)70081-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199707)172:1<12::AID-JCP2>3.0.CO;2-G"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/315682"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3602-3610.2002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00249-1"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.112.1.235"},{"key":"e_1_2_7_10_1","first-page":"790","article-title":"Pharmacokinetics of aminoguanidine administration and effects on the diabetes frequency in nonobese diabetic mice","volume":"279","author":"BOWMAN M.A.","year":"1996","journal-title":"J. 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