{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T19:28:20Z","timestamp":1747769300964},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2438,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effect of trichloroethanol (TCEt), the active metabolite of chloral hydrate, on the intracellular concentration of calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) was investigated in rat submandibular glands (RSMG) acini loaded with fura\u20102.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>TCEt (1\u201310\u2003m<jats:sc>M<\/jats:sc>) increased the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> independently of the presence of calcium in the extracellular medium. Dichloroethanol (DCEt) and monochloroethanol (MCEt) reproduced the stimulatory effect of TCEt but at much higher concentrations (about 6 fold higher for DCEt and 20 fold higher for MCEt).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>TCEt mobilized an intracellular pool of calcium, which was depleted by a pretreatment with thapsigargin, an inhibitor of the sarcoplasmic and endoplasmic reticulum calcium\u2010dependent ATPases, but not with FCCP, an uncoupler of mitochondria.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>TCEt 10\u2003m<jats:sc>M<\/jats:sc> inhibited by 50% the thapsigargin\u2010sensitive microsomal Ca<jats:sup>2+<\/jats:sup>\u2010ATPase. DCEt 10\u2003m<jats:sc>M<\/jats:sc> and MCEt 10\u2003m<jats:sc>M<\/jats:sc> inhibited the ATPase by 20 and 10%, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>TCEt inhibited the increase of the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and the production of inositol phosphates in response to carbachol, epinephrine and substance P.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>TCEt inhibited the uptake of calcium mediated by the store\u2010operated calcium channel (SOCC).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>ATP and Bz\u2010ATP increased the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in RSMG acini and this effect was blocked by extracellular magnesium, by Coomassie blue and by oxydized ATP (oATP).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>TCEt potentiated the increase of the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and of the uptake of extracellular calcium in response to ATP and Bz\u2010ATP.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>TCEt had no effect on the uptake of barium and of ethidium bromide in response to purinergic agonists.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results suggest that TCEt, at sedative concentrations, exerts various effects on the calcium regulation: (1) it mobilizes a thapsigargin\u2010sensitive intracellular pool of calcium in RSMG acini; (2) it inhibits the uptake of calcium <jats:italic>via<\/jats:italic> the SOCC; (3) it inhibits the activation by G protein\u2010coupled receptors of a polyphosphoinositide\u2010specific phospholipase C. It does not interfere with the activation of the ionotropic P2X receptors.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The use of chloral hydrate should be avoided in studies exploring the <jats:italic>in vivo<\/jats:italic> responses to sialagogues.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>136<\/jats:bold>, 568\u2013580; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704745\">10.1038\/sj.bjp.0704745<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704745","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T22:11:23Z","timestamp":1106950283000},"page":"568-580","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multiple effects of trichloroethanol on calcium handling in rat submandibular acinar cells"],"prefix":"10.1111","volume":"136","author":[{"given":"S","family":"Pochet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Keskiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Fernandez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Marino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Cha\u00efb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J P","family":"Dehaye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"M\u00e9tioui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1340","article-title":"Pharmacokinetic analysis of chloral hydrate and its metabolism in B6C3F1 mice","volume":"24","author":"ABBAS R.R.","year":"1996","journal-title":"Drug Metab. Dispos."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411000110010301"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00437-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4274(91)90073-F"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9627323"},{"key":"e_1_2_7_8_1","first-page":"35","volume-title":"Drug and Anesthetic Effects on Membrane Structure and Function","author":"CURATOLA G.","year":"1991"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703124"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(99)90014-6"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.4049\/jimmunol.143.6.1955","article-title":"Responses of mouse lymphocytes to extracellular adenosine 5\u2032\u2010triphosphate (ATP)","volume":"143","author":"DI VIRGILIO F.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018073"},{"key":"e_1_2_7_13_1","volume-title":"Biomembrane Protocols, Methods in Molecular Biology","author":"EAST J.M.","year":"1993"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1998.8486"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/274339a0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1054\/ceca.2000.0169"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.2307\/1536405"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00833-5"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107794200"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)40103-8"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.25.15771"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.17.8200"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(86)90298-1"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi970028w"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(99)00164-1"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(99)00111-0"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411990100020601"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(94)90159-7"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1906.0001"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53082-9"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1991.tb09217.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703347"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.40.1.563"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(93)90079-7"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(96)10003-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000765"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00924-8"},{"key":"e_1_2_7_39_1","first-page":"210","article-title":"A review of laboratory animal anesthesia with chloral hydrate and chloralose","volume":"43","author":"SILVERMAN J.","year":"1993","journal-title":"Lab. Anim. Sci."},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03159978"},{"key":"e_1_2_7_41_1","doi-asserted-by":"crossref","first-page":"C194","DOI":"10.1152\/ajpcell.1991.260.2.C194","article-title":"NaF\u2010induced amylase release from rat parotid cells is mediated by PI breakdown leading to Ca2+ mobilization","volume":"260","author":"TOJYO Y.","year":"1991","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_42_1","first-page":"1","volume-title":"Drug and Anesthetic Effects on Membrane Structure and Function","author":"TRUDELL J.R.","year":"1991"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.2.481"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1392"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.41.1.23"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704745","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0704745","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0704745","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T13:52:06Z","timestamp":1697550726000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0704745"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":44,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["10.1038\/sj.bjp.0704745"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0704745","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]},"assertion":[{"value":"2009-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}