{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:25:02Z","timestamp":1771626302827,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2316,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item>\n<jats:p>The present study has investigated mechanisms underlying ATP\u2010induced endothelium\u2010independent enhancement of vasomotion in guinea\u2010pig mesenteric lymphatic vessels.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Lymphatic vasomotion, vessel tone and smooth muscle [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> showed similar ATP concentration\u2010response curves.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>ATP, at 0.1 m<jats:sc>M<\/jats:sc>, caused a biphasic increase in tonic [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and superimposed vasomotion\u2010associated Ca<jats:sup>2+<\/jats:sup> transients. All ATP\u2010induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> changes were abolished by incubating the smooth muscle with suramin (0.1 m<jats:sc>M<\/jats:sc>).<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>\u03b1,\u03b2\u2010MeATP (0.1 m<jats:sc>M<\/jats:sc>) and UTP (0.1 m<jats:sc>M<\/jats:sc>) caused similar changes in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> but the responses to these agonists were smaller than to ATP.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>The actions of \u03b1,\u03b2\u2010MeATP (0.1 m<jats:sc>M<\/jats:sc>) were inhibited by suramin (0.1 m<jats:sc>M<\/jats:sc>) and PPADS (30 \u03bc<jats:sc>M<\/jats:sc>) but not by reactive blue 2 (30 \u03bc<jats:sc>M<\/jats:sc>).<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>In the presence of \u03b1,\u03b2\u2010MeATP (0.1 m<jats:sc>M<\/jats:sc>), the increases in tonic [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and vasomotion\u2010associated Ca<jats:sup>2+<\/jats:sup> transients induced by ATP (0.1 m<jats:sc>M<\/jats:sc>) were inhibited by U73122 (5 \u03bc<jats:sc>M<\/jats:sc>), CPA (20 \u03bc<jats:sc>M<\/jats:sc>) and heparin, whereas U73343 (5 \u03bc<jats:sc>M<\/jats:sc>) and pre\u2010treatment with PTx (100 ng ml<jats:sup>\u22121<\/jats:sup>) had no significant effects.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Depletion of the intracellular stores with CPA (20 \u03bc<jats:sc>M<\/jats:sc>) caused an increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, which was not blocked by desensitization of P<jats:sub>2X<\/jats:sub> receptors with \u03b1,\u03b2\u2010MeATP.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>The data indicate that ATP, at relatively high concentrations increases lymphatic smooth muscle [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and vasomotion through activation of P<jats:sub>2X1<\/jats:sub> and P<jats:sub>2Y2<\/jats:sub> purinoceptors present on lymphatic smooth muscle. The increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> is likely to result from Ca<jats:sup>2+<\/jats:sup> release from inositol\u20101,4,5\u2010trisphosphate\u2010sensitive stores as well as Ca<jats:sup>2+<\/jats:sup> influx through store\u2010operated channels and P<jats:sub>2X<\/jats:sub>\u2010gated channels.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>137<\/jats:bold>, 477\u2013487. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704899\">10.1038\/sj.bjp.0704899<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704899","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T17:41:09Z","timestamp":1033494069000},"page":"477-487","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["ATP\u2010induced endothelium\u2010independent enhancement of lymphatic vasomotion in guinea\u2010pig mesentery involves P<sub>2X<\/sub> and P<sub>2Y<\/sub> receptors"],"prefix":"10.1111","volume":"137","author":[{"given":"Jun","family":"Zhao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk F","family":"van Helden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"756","article-title":"Selective inhibition of receptor\u2010coupled phospholipase C\u2010dependent processes in human platelets and polymorphonuclear neutrophils","volume":"255","author":"BLEASDALE J.E.","year":"1990","journal-title":"J. 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