{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:03:28Z","timestamp":1791198208152,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2255,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Cyclo\u2010oxygenase (COX) and lipoxygenase (LO) share a common substrate, arachidonic acid. Aspirin and related drugs inhibit COX activity. In a subset of patients with asthma aspirin induces clinical symptoms associated with increased levels of certain LO products, a phenomenon known as aspirin\u2010sensitive asthma. The pharmacological pathways regulating such responses are not known.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Here COX\u20101 and LO activity were measured respectively by the formation of thromboxane B<jats:sub>2<\/jats:sub> (TXB<jats:sub>2<\/jats:sub>) or leukotrienes (LT) C<jats:sub>4<\/jats:sub>, D<jats:sub>4<\/jats:sub> and E<jats:sub>4<\/jats:sub> in whole blood stimulated with A23187. COX\u20102 activity was measured by the formation of prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) in blood stimulated with lipopolysaccharide (LPS) for 18 h.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>No differences in the levels of COX\u20101, COX\u20102 or LO products or the potency of drugs were found in blood from aspirin sensitive vs aspirin tolerant patients. Aspirin, indomethacin and nimesulide inhibited COX\u20101 activity, without altering LO activity. Indomethacin, nimesulide and the COX\u20102 selective inhibitor DFP [5,5\u2010dimethyl\u20103\u2010(2\u2010isopropoxy)\u20104\u2010(4\u2010methanesulfonylphenyl)\u20102(5<jats:italic>H<\/jats:italic>)\u2010furanone] inhibited COX\u20102 activity. NO\u2010aspirin, like aspirin inhibited COX\u20101 activity in blood from both groups. However, NO\u2010aspirin also reduced LO activity in the blood from both patient groups. Sodium salicylate was an ineffective inhibitor of COX\u20101, COX\u20102 or LO activity in blood from both aspirin\u2010sensitive and tolerant patients.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Thus, when COX activity in the blood of aspirin\u2010sensitive asthmatics is blocked there is no associated increase in LO products. Moreover, NO\u2010aspirin, unlike other NSAIDs tested, inhibited LO activity in the blood from both aspirin sensitive and aspirin tolerant individuals. This suggests that NO\u2010aspirin may be better tolerated than aspirin by aspirin\u2010sensitive asthmatics.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>137<\/jats:bold>, 1031\u20131038. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704927\">10.1038\/sj.bjp.0704927<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704927","type":"journal-article","created":{"date-parts":[[2002,11,12]],"date-time":"2002-11-12T18:47:25Z","timestamp":1037126845000},"page":"1031-1038","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Effects of non\u2010steroidal anti\u2010inflammatory drugs on cyclo\u2010oxygenase and lipoxygenase activity in whole blood from aspirin\u2010sensitive asthmatics vs healthy donors"],"prefix":"10.1111","volume":"137","author":[{"given":"P A","family":"Gray","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T D","family":"Warner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Vojnovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Del Soldato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Parikh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G K","family":"Scadding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J A","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.118.5.1470"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702104"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0700963"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(97)00136-2"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(01)00478-2"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(91)90328-L"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.21.9475"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.7.3592"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)90909-0"},{"key":"e_1_2_6_11_1","first-page":"P1","article-title":"Leukotriene production in human blood is reduced by NO\u2010aspirin but not aspirin: comparisons between responses in healthy volunteers and aspirin\u2010sensitive asthma","volume":"129","author":"GRAY P.A.","year":"2000","journal-title":"Br. 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