{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T19:17:34Z","timestamp":1720725454284},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2251,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item>\n<jats:p>Distension\u2010sensitive vagal afferent fibres from the guinea\u2010pig oesophagus were recorded extracellularly <jats:italic>in vitro<\/jats:italic>. Most recorded units were spontaneously active firing at 3.2\u00b10.3 Hz (<jats:italic>n<\/jats:italic>=41, <jats:italic>N<\/jats:italic>=41) and had low thresholds (less than 1 mm) to circumferential stretch. Dynamic and adapted phases of stretch\u2010evoked firing, as well as a silent period were linearly dependent on the amplitude of stretch.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>High K<jats:sup>+<\/jats:sup> (7\u201312 m<jats:sc>M<\/jats:sc>) Krebs solution dose\u2010dependently increased both spontaneous and stretch\u2010evoked firing and reduced the duration of the silent period.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Charybdotoxin (ChTX, 100 n<jats:sc>M<\/jats:sc>) slightly increased spontaneous and stretch\u2010evoked firing and decreased the silent period, while neither iberiotoxin (100 n<jats:sc>M<\/jats:sc>) nor apamin (0.5 \u03bc<jats:sc>M<\/jats:sc>) had significant effects. \u03c9\u2010Conotoxin GVIA (0.5 \u03bc<jats:sc>M<\/jats:sc>) did not significantly affect firing of vagal mechanoreceptors.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>In the majority of single units, 4\u2010aminopyridine (4\u2010AP) concentration\u2010dependently (EC<jats:sub>50<\/jats:sub>\u223c28 \u03bc<jats:sc>M<\/jats:sc>) increased spontaneous firing, strongly reduced the silent period but did not affect stretch (3 mm)\u2010induced firing. Firing evoked by 1\u20132 mm was increased by 4\u2010AP.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>\u03b1\u2010Dendrotoxin (DnTX, 300 n<jats:sc>M<\/jats:sc>) and DnTX K (30 n<jats:sc>M<\/jats:sc>) slightly increased spontaneous and stretch\u2010evoked firing. There was no additive effect on spontaneous firing when ChTX and DnTX K were applied simultaneously.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>Barium (100 \u03bc<jats:sc>M<\/jats:sc>) increased stretch\u2010induced firing, probably due to an increase in intramural tension. Glibenclamide (10 \u03bc<jats:sc>M<\/jats:sc>) had no effect on spontaneous or stretch\u2010induced firing.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>The results indicate that voltage\u2010gated 4\u2010AP\u2010 and dendrotoxin\u2010sensitive K<jats:sup>+<\/jats:sup> channels are the main type of K<jats:sup>+<\/jats:sup> channels that influence excitability of vagal mechano\u2010sensitive endings of the guinea\u2010pig oesophagus. They were involved in control of spontaneous firing and in stretch\u2010induced firing evoked by moderate stretch, but none of the K<jats:sup>+<\/jats:sup> channels appeared to be involved in adaptation to maintained stretch by their slowly adapting vagal mechanoreceptors.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>137<\/jats:bold>, 1195\u20131206. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704964\">10.1038\/sj.bjp.0704964<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704964","type":"journal-article","created":{"date-parts":[[2002,12,4]],"date-time":"2002-12-04T18:22:03Z","timestamp":1039026123000},"page":"1195-1206","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["4\u2010aminopyridine\u2010 and dendrotoxin\u2010sensitive potassium channels influence excitability of vagal mechano\u2010sensitive endings in guinea\u2010pig oesophagus"],"prefix":"10.1111","volume":"137","author":[{"given":"Vladimir P","family":"Zagorodnyuk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bao Nan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcello","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon J H","family":"Brookes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(93)90480-6"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2028"},{"key":"e_1_2_7_4_1","first-page":"528","article-title":"Initiation of peristalsis by circumferential stretch in flat sheets of guinea\u2010pig ileum","volume":"516","author":"BROOKES S.J.H.","year":"1999","journal-title":"J. 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