{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:30:42Z","timestamp":1791401442186,"version":"4.3.3"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":2255,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The action of the main ciguatoxin involved in ciguatera fish poisoning in the Pacific region (P\u2010CTX\u20101b) was studied in myotubes originated from rat skeletal muscle cells kept in primary culture.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The effect of P\u2010CTX\u20101b on sodium currents at short times of exposure (up to 1 min) showed a moderate increase in peak Na<jats:sup>+<\/jats:sup> current. During prolonged exposures, P\u2010CTX\u20101b decreased the peak Na<jats:sup>+<\/jats:sup> current. This action was always accompanied by an increase of leakage currents, tail currents and outward Na<jats:sup>+<\/jats:sup> currents, resulting in an intracellular Na<jats:sup>+<\/jats:sup> accumulation. This effect is blocked by prior exposure to tetrodotoxin (TTX) and becomes evident only after washout of TTX.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Low to moderate concentrations of P\u2010CTX\u20101b (2\u20135 n<jats:sc>M<\/jats:sc>) partially blocked potassium currents in a manner that was dependent on the membrane potential.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>P\u2010CTX\u20101b (2\u201312 n<jats:sc>M<\/jats:sc>) caused a small membrane depolarization (3\u20135 mV) and an increase in the frequency of spontaneous action potential discharges that reached in general low frequencies (0.1\u20130.5 Hz).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>P\u2010CTX\u20101b (10 n<jats:sc>M<\/jats:sc>) caused a transient increase of intracellular inositol 1,4,5\u2010trisphosphate (IP<jats:sub>3<\/jats:sub>) mass levels, which was blocked by TTX.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the presence of P\u2010CTX\u20101b (10 n<jats:sc>M<\/jats:sc>) and in the absence of external Ca<jats:sup>2+<\/jats:sup>, the intracellular Ca<jats:sup>2+<\/jats:sup> levels show a transient increase in the cytoplasm as well as in the nuclei. The time course of this effect may reflect the action of IP<jats:sub>3<\/jats:sub> over internal stores activated by P\u2010CTX\u20101b\u2010induced membrane depolarization.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2002) <jats:bold>137<\/jats:bold>, 1055\u20131062. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0704980\">10.1038\/sj.bjp.0704980<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0704980","type":"journal-article","created":{"date-parts":[[2002,11,12]],"date-time":"2002-11-12T18:47:25Z","timestamp":1037126845000},"page":"1055-1062","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Pacific ciguatoxin\u20101b effect over Na<sup>+<\/sup> and K<sup>+<\/sup> currents, inositol 1,4,5\u2010triphosphate content and intracellular Ca<sup>2+<\/sup> signals in 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