{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:37:17Z","timestamp":1792121837641,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2100,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The venom of <jats:italic>Phoneutria nigriventer<\/jats:italic> spider (PNV) causes intense pain and inflammation following an attack. We have investigated the involvement of capsaicin\u2010sensitive nerve fibres by utilizing an <jats:italic>in vitro<\/jats:italic> nerve preparation. Extracellular DC potential recordings were made from the rat\u2010isolated vagus nerve, a preparation that is rich in capsaicin\u2010sensitive, that is, nociceptive, C\u2010fibres.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>PNV (1\u201310 <jats:italic>\u03bc<\/jats:italic>g ml<jats:sup>\u22121<\/jats:sup>), capsaicin (0.03\u20130.3 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) or 5\u2010hydroxytriptamine (5\u2010HT) (0.3\u20133 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) induced dose\u2010dependent depolarizations of vagus nerve fibres. Depolarizing responses to capsaicin were blocked by ruthenium red (RR, 10 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>), but responses to PNV were not. Depolarizing responses to PNV or veratridine (50 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) were inhibited by tetrodotoxin (TTX, 10 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>), but those to capsaicin were not. This suggests that capsaicin and PNV depolarize the nerve fibres by distinct mechanisms.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Depolarization in response to 5\u2010HT (3 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) was reduced by the 5\u2010HT<jats:sub>3<\/jats:sub> receptor antagonists Y25130 (0.5 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) and tropisetron (10 n<jats:sc>M<\/jats:sc>) or, to a lesser extent, by the 5\u2010HT<jats:sub>4<\/jats:sub> receptor antagonist RS39604 (1 or 10 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>). Depolarizing responses to PNV were not affected significantly by Y25130 or tropisetron, but were blocked by RS39604.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data show that 5\u2010HT<jats:sub>4<\/jats:sub> receptors play a significant role in the activation of nociceptive sensory nerve fibres by PNV and suggest that this is of importance in the development of the pain and inflammation associated with bites from the <jats:italic>P. nigriventer<\/jats:italic> spider.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2003) <jats:bold>139<\/jats:bold>, 59\u201364. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705240\">10.1038\/sj.bjp.0705240<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705240","type":"journal-article","created":{"date-parts":[[2003,5,13]],"date-time":"2003-05-13T20:08:44Z","timestamp":1052856524000},"page":"59-64","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["<i>Phoneutria nigriventer<\/i> spider venom activates 5\u2010HT<sub>4<\/sub> receptors in rat\u2010isolated vagus nerve"],"prefix":"10.1111","volume":"139","author":[{"given":"Soraia K P","family":"Costa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan D","family":"Brain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson","family":"Antunes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto","family":"De Nucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reginald J","family":"Docherty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(92)90045-7"},{"key":"e_1_2_7_3_1","first-page":"205","article-title":"Effects of a toxic fraction, PhTx2, from the spider Phoneutria nigriventer on the sodium current","volume":"347","author":"ARAUJO D.A.","year":"1993","journal-title":"Naunyn Schmiedebergs Arch. 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