{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:16:34Z","timestamp":1789514194964,"version":"build-2803163510"},"reference-count":70,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":2069,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Studies were undertaken to determine the nature of the receptors mediating contractile effects of tachykinins in the uteri of nonpregnant women, and to analyse the expression of preprotachykinins (PPT), tachykinin receptors and the cell\u2010surface peptidase, neprilysin (NEP), in the myometrium from pregnant and nonpregnant women.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The neurokinin B (NKB) precursor PPT\u2010B was expressed in higher levels in the myometrium from nonpregnant than from pregnant women. Faint expression of PPT\u2010A mRNA was detectable in the myometrium from nonpregnant but not pregnant women. PPT\u2010C, the gene encoding the novel tachykinin peptide hemokinin\u20101 (HK\u20101), was present in trace amounts in the uteri from both pregnant and nonpregnant women.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Tachykinin NK<jats:sub>2<\/jats:sub> receptors were more strongly expressed in tissues from nonpregnant than from pregnant women. NK<jats:sub>1<\/jats:sub> receptor mRNA was present in low levels in tissues from both pregnant and nonpregnant women. A low abundance transcript corresponding to the NK<jats:sub>3<\/jats:sub> receptor was present only in tissues from nonpregnant women.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The mRNA expression of the tachykinin\u2010degrading enzyme NEP was lower in tissues from nonpregnant than from pregnant women.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Substance P (SP), neurokinin A (NKA) and NKB, in the presence of the peptidase inhibitors thiorphan, captopril and bestatin, produced contractions of myometrium from nonpregnant women. The order of potency was NKA\u226bSP\u2265NKB. The potency of NKA was unchanged in the absence of peptidase inhibitors.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The tachykinin NK<jats:sub>2<\/jats:sub> receptor\u2010selective agonist [Lys<jats:sup>5<\/jats:sup>MeLeu<jats:sup>9<\/jats:sup>Nle<jats:sup>10<\/jats:sup>]NKA(4\u2013l0) was approximately equipotent with NKA, but the tachykinin NK<jats:sub>1<\/jats:sub> and NK<jats:sub>3<\/jats:sub> receptor\u2010selective agonists [Sar<jats:sup>9<\/jats:sup>Met(O<jats:sub>2<\/jats:sub>)<jats:sup>11<\/jats:sup>]SP and [MePhe<jats:sup>7<\/jats:sup>]NKB were ineffective in the myometrium from nonpregnant women.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The uterotonic effects of [Lys<jats:sup>5<\/jats:sup>MeLeu<jats:sup>9<\/jats:sup>Nle<jats:sup>10<\/jats:sup>]NKA(4\u201310) were antagonized by the tachykinin NK<jats:sub>2<\/jats:sub> receptor\u2010selective antagonist SR48968. Neither atropine, nor phentolamine nor tetrodotoxin affected responses to [Lys<jats:sup>5<\/jats:sup>MeLeu<jats:sup>9<\/jats:sup>Nle<jats:sup>10<\/jats:sup>]NKA(4\u201310).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data are consistent with a role of tachykinins in the regulation of human uterine function, and reinforce the importance of NK<jats:sub>2<\/jats:sub> receptors in the regulation of myometrial contraction.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2003) <jats:bold>139<\/jats:bold>, 523\u2013532. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705279\">10.1038\/sj.bjp.0705279<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705279","type":"journal-article","created":{"date-parts":[[2003,6,4]],"date-time":"2003-06-04T23:44:34Z","timestamp":1054770274000},"page":"523-532","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Tachykinins and tachykinin receptors in human uterus"],"prefix":"10.1111","volume":"139","author":[{"given":"Eva","family":"Patak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Luz Candenas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jocelyn N","family":"Pennefather","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Ziccone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison","family":"Lilley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio D","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Flores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio G","family":"Mantec\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margot E","family":"Story","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco M","family":"Pinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(78)90044-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00226501"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1995.sp003874"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod53.4.814"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S1071-5576(01)00145-9","article-title":"Pelvic nerve plexus trauma at radical and simple hysterectomy: a quantitative study of nerve types in the uterine supporting ligaments","volume":"9","author":"BUTTLER\u2010MANUEL S.A.","year":"2002","journal-title":"J. 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