{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:07:22Z","timestamp":1790370442041,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,2]],"date-time":"2009-02-02T00:00:00Z","timestamp":1233532800000},"content-version":"vor","delay-in-days":1890,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The aim of this work was to study the effects of <jats:italic>N<\/jats:italic>\u2010salicyloyltryptamine (STP), a novel anticonvulsant agent, on voltage\u2010gated ion channels in GH3 cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In this study, we show that STP at 17 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc> inhibited up to 59.2\u00b110.4% of the <jats:italic>I<\/jats:italic><jats:sub>to<\/jats:sub> and 73.1\u00b18.56% of the <jats:italic>I<\/jats:italic><jats:sub>KD<\/jats:sub> K<jats:sup>+<\/jats:sup> currents in GH3 cells. Moreover, the inhibitory activity of the drug STP on K<jats:sup>+<\/jats:sup> currents was dose\u2010dependent (IC<jats:sub>50<\/jats:sub>=34.6\u00b18.14 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc> for <jats:italic>I<\/jats:italic><jats:sub>to<\/jats:sub>) and partially reversible after washing off.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Repeated stimulation at 1 Hz (STP at 17 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) led to the total disappearance of <jats:italic>I<\/jats:italic><jats:sub>to<\/jats:sub> current, and an enhancement of <jats:italic>I<\/jats:italic><jats:sub>KD<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the cell\u2010attached configuration, application of STP to the bath increased the open probability of large\u2010conductance Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> channels.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>STP at 17 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc> inhibited the L\u2010type Ca<jats:sup>2+<\/jats:sup> current by 54.9\u00b17.50% without any significant changes in the voltage dependence.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>STP at 170 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc> inhibited the TTX\u2010sensitive Na<jats:sup>+<\/jats:sup> current by 22.1\u00b12.41%. At a lower concentration (17 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>), no effect on <jats:italic>I<\/jats:italic><jats:sub>Na<\/jats:sub> was observed.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The pharmacological profile described here might contribute to the neuroprotective effect exerted by this compound in experimental \u2018<jats:italic>in vivo<\/jats:italic>\u2019 models.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2003) <jats:bold>140<\/jats:bold>, 1331\u20131339. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705471\">10.1038\/sj.bjp.0705471<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705471","type":"journal-article","created":{"date-parts":[[2003,11,26]],"date-time":"2003-11-26T18:51:37Z","timestamp":1069872697000},"page":"1331-1339","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<i>N<\/i>\u2010Salicyloyltryptamine, a new anticonvulsant drug, acts on voltage\u2010dependent Na<sup>+<\/sup>, Ca<sup>2+<\/sup>, and K<sup>+<\/sup> ion channels"],"prefix":"10.1111","volume":"140","author":[{"given":"Dem\u00e9trius Antonio Machado","family":"Ara\u00fajo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta Amaral","family":"Mafra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9ia Laura Prates","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e1lter","family":"Miguel\u2010Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo S\u00e9rgio Lacerda","family":"Beir\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinaldo N\u00f3brega","family":"De Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Lucindo","family":"Quintans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria F\u00e1tima Vanderlei de","family":"Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jader Santos","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1982.sp014370"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020019"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.70.5.549"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1528-1157.2001.43600.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.109.5.647"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002100000341"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002100100408"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(01)02169-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02863-3"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)60954-0"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703447"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000539-200105000-00021"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00656997"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00349.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02110699"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(01)00122-X"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(99)00267-1"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1157.2000.tb00301.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-1775-1_7"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02262-4"},{"key":"e_1_2_7_22_1","first-page":"355","article-title":"Methylphenylethynylpyridine (MPEP) Novartis","volume":"1","author":"MICHELI F.","year":"2000","journal-title":"Curr. 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