{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T22:11:43Z","timestamp":1780611103714,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T00:00:00Z","timestamp":1233273600000},"content-version":"vor","delay-in-days":1825,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The roles of nitric oxide (NO), superoxide anion (O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>), and hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) in the modulation of spontaneous tone were investigated in isolated aorta from deoxycorticosterone acetate (DOCA)\u2010salt hypertensive rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Increases in preload from 1 to 5 g were accompanied by increases in spontaneous tone in aortic rings from DOCA\u2010salt hypertensive rats but not from SHAM\u2010normotensive rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Tone was higher in endothelium\u2010denuded aortic rings than in endothelium\u2010intact vessels. Inhibition of nitric oxide synthase (NOS) with 300 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc> <jats:italic>N<\/jats:italic><jats:sup><jats:italic>G<\/jats:italic><\/jats:sup>\u2010nitro\u2010<jats:sc>L<\/jats:sc>\u2010arginine methyl ester (<jats:sc>L<\/jats:sc>\u2010NAME) increased spontaneous tone.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Basal O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> generation was higher in aortic rings from DOCA\u2010salt hypertensive rats than in those from SHAM\u2010normotensive rats. Stretch increased O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> levels even further in the DOCA\u2010salt group. In rings isolated from DOCA\u2010salt hypertensive rats, administration of the O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> scavenger, superoxide dismutase (SOD, 150 U ml<jats:sup>\u22121<\/jats:sup>), or the nicotinamide adenine dinucleotide phosphate (NADPH)\u2010oxidase inhibitor, apocynin (100 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>), completely abolished the development of spontaneous tone in endothelium\u2010intact aortic rings but not in endothelium\u2010denuded or in <jats:sc>L<\/jats:sc>\u2010NAME\u2010treated rings. SOD and apocynin decreased the generation of O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> in endothelium\u2010intact, endothelium\u2010denuded, and <jats:sc>L<\/jats:sc>\u2010NAME\u2010treated aortic rings.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Oral treatment of DOCA\u2010salt hypertensive rats with the O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> scavengers, tempol or tiron, or with apocynin for 3 weeks prevented the development of hypertension and abolished the increases in O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> generation and spontaneous tone.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Administration of catalase (1000 U ml<jats:sup>\u22121<\/jats:sup>) to aortic rings increased spontaneous tone in vessels from DOCA\u2010salt hypertensive rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Administration of the cyclooxygenase (COX) inhibitor, valeroyl salicylate, or the thromboxane\/prostaglandin antagonist, SQ 29548, to aortic rings abolished tone.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results suggest that NO plays a major role in preventing the generation of spontaneous tone in isolated aortic rings from DOCA\u2010salt hypertensive rats. NADPH\u2010oxidase\u2010derived O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> enhanced spontaneous tone by inactivating NO. Endogenous H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> appears to mitigate the increase in tone. In addition, a COX component may also contribute to spontaneous tone.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>141<\/jats:bold>, 562\u2013573. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705557\">10.1038\/sj.bjp.0705557<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705557","type":"journal-article","created":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T18:17:58Z","timestamp":1077214678000},"page":"562-573","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Role of oxidative stress and nitric oxide in regulation of spontaneous tone in aorta of DOCA\u2010salt hypertensive rats"],"prefix":"10.1111","volume":"141","author":[{"given":"Mahua","family":"Ghosh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui Di","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Robert","family":"McNeill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1902.sp000911"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/hy1101.093423"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.37.2.781"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01076587"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.18.2.132"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/327717a0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.33.5.1225"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1139\/y02-106"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.74.6.1141"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02750638"},{"key":"e_1_2_7_12_1","first-page":"H87","article-title":"Stretch\u2010dependent (myogenic) tone in rabbit ear resistance arteries","volume":"250","author":"HWA J.J.","year":"1986","journal-title":"Am. 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